JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100777

Rat MCP1 ELISA Kit (CCL2)

Be the first to review this product! Submit a review

|

(4 Publications)

Rat MCP1 ELISA Kit (CCL2) is a Sandwich ELISA for the measurement of Rat MCP1 (CCL2) in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1

2 Images
Sandwich ELISA - Rat MCP1 ELISA Kit (CCL2) (AB100777)
  • sELISA

Unknown

Sandwich ELISA - Rat MCP1 ELISA Kit (CCL2) (AB100777)

Representative standard curve using ab100777

Sandwich ELISA - Rat MCP1 ELISA Kit (CCL2) (AB100777)
  • sELISA

Unknown

Sandwich ELISA - Rat MCP1 ELISA Kit (CCL2) (AB100777)

Representative standard curve using ab100777

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 15 pg/mL

Range

24.67 - 18000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB214566

Rat IL-10 ELISA Kit

Recombinant|SimpleStep

ELISA - Rat IL-10 ELISA Kit (AB214566)

  • 0
  • 0
Assay Kits

AB236712

Rat TNF alpha ELISA Kit

Recombinant|SimpleStep

ELISA - Rat TNF alpha ELISA Kit (AB236712)

  • 0
  • 0
Assay Kits

AB255730

Rat IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Rat IL-1 beta ELISA Kit (AB255730)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP1 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 105 %", "average": "= 101.4" }, { "sample": "Serum", "range": "84 - 103 %", "average": "= 95.39" }, { "sample": "Plasma", "range": "83 - 103 %", "average": "= 92.44" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100777-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100777-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100777-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB100777-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat MCP1", "number":"AB100777-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100777-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MCP1 Microplate (12 x 8 wells)", "number":"AB100777-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100777-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100777-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat MCP1 Standard (lyophilized)", "number":"AB100777-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
style
left-column

Product protocols

style
left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
See full target information Ccl2
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical biology 59:1607-1618 PubMed34818128

2021

Protective effect of Qingre Huoxue decoction against myocardial infarction via PI3K/Akt autophagy pathway based on UPLC-MS, network pharmacology, and evidence.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Jin,Wenbo Zhang,Yuan Luo,Xiushen Li,Lijin Qing,Qiang Zuo,Junfeng Fang,Wei Wu

Cellular physiology and biochemistry : internation 46:375-388 PubMed29590655

2018

Lipocalin 2 Suppresses Ocular Inflammation by Inhibiting the Activation of NF-κβ Pathway in Endotoxin-Induced Uveitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyi Tang,Jun Ma,Ruiping Gu,Xinyi Ding,Boya Lei,Xin Wang,Hong Zhuang,Gezhi Xu

Experimental eye research 165:78-89 PubMed28888911

2017

Photobiomodulation with 670 nm light ameliorates Müller cell-mediated activation of microglia and macrophages in retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Zhen Lu,Riccardo Natoli,Michele Madigan,Nilisha Fernando,Kartik Saxena,Riemke Aggio-Bruce,Haihan Jiao,Jan Provis,Krisztina Valter

Planta medica 80:870-9 PubMed25116118

2014

Ruscogenin protects against high-fat diet-induced nonalcoholic steatohepatitis in hamsters.

Applications

Unspecified application

Species

Unspecified reactive species

Hung-Jen Lu,Shorong-Shii Liou,Chia Ju Chang,Sheng Da Lin,Cheng Yang,Ming-Chang Wu,I-Min Liu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com