AB327325
Rat MCP1 ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- What is this?
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Rat MCP1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Rat MCP1 with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.47 - 1800 pg/ml with a sensitivity of 1.96 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.47 - 1800 pg/ml with a sensitivity of 1.96 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
3 Images
- sELISA
Supplier Data
Sandwich ELISA - Rat MCP1 ELISA Kit, Chemiluminescent (AB327325)
Interpolated concentrations of native MCP1 in rat serum plasma and cell culture supernatant samples.
The concentrations of MCP1 were measured in duplicates interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 7.5% plasma (citrate) 50% and spleen supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The MCP1 concentration was determined to be 5485 pg/mL in serum 1615 pg/mL in plasma (citrate) and 40501 pg/mL in and spleen supernatant.
- sELISA
Lab
Sandwich ELISA - Rat MCP1 ELISA Kit, Chemiluminescent (AB327325)
Standard curve comparison between the Chemiluminescent Rat MCP1 SimpleStep ELISA and colorimetric Rat MCP1 SimpleStep ELISA (ab219045) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Rat MCP1 ELISA Kit, Chemiluminescent (AB327325)
Dynamic range comparison between the Chemiluminescent Rat MCP1 SimpleStep ELISA, the colorimetric Rat MCP1 SimpleStep ELISA (ab219045), and the leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows an increased dynamic range.
Reactivity data
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Product details
Rat MCP1 ELISA Kit, Chemiluminescent (ab327325) is a rapid single-wash sandwich 55-min ELISA to measure Rat MCP1 in Cell culture supernatant, Serum, Citrate plasma with a sensitivity of 1.96 pg/mL.
How the assay works
Rat MCP1 ELISA Kit, Chemiluminescent (ab327325) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Rat MCP1 (ab327325) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-rt_MCP1 antibody [EPR19906-201] - BSA and Aside free (Capture) ab242665
- Anti-rt_MCP1 antibody [EPR19906-218] - BSA and Aside free (Detector) ab242910
How the assay works
Rat MCP1 ELISA Kit, Chemiluminescent (ab327325) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Rat MCP1 (ab327325) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-rt_MCP1 antibody [EPR19906-201] - BSA and Aside free (Capture) ab242665
- Anti-rt_MCP1 antibody [EPR19906-218] - BSA and Aside free (Detector) ab242910
Precision
[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]
Recovery
[ { "sample": "Cell culture supernatant", "range": "88 - 98 %", "average": "= 93" }, { "sample": "Serum", "range": "109 - 113 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "94 - 107 %", "average": "= 101" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
Alternative Names
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1
Product promise
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