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AB213916

Rat MIP-1 alpha/CCL3 ELISA Kit

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(2 Publications)

Rat MIP-1 alpha/CCL3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat MIP-1 alpha/CCL3 in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, Small-inducible cytokine A3, MIP-1-alpha

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Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB213916)
  • sELISA

Supplier Data

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB213916)

MIP-1 alpha ELISA Kit (ab213916) Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 1 pg/mL

Range

7.8 - 500 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The MIP-1alpha Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213916) is designed for the quantitative measurement of MIP-1 alpha in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-1 alpha has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for MIP-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MIP-1 alpha amount of sample captured in plate.

Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "267 pg/mL", "standardDeviation": "15.75", "coefficientOfVariability": "= 5.9" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "18 pg/mL", "standardDeviation": "1.08", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "56 pg/mL", "standardDeviation": "4.48", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "54 pg/mL", "standardDeviation": "3.61", "coefficientOfVariability": "= 6.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "248 pg/mL", "standardDeviation": "14.88", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "18 pg/mL", "standardDeviation": "1.11", "coefficientOfVariability": "= 6.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213916-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213916-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- rat MIP-1 alpha/CCL3 antibody", "number":"AB213916-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-rat MIP-1 alpha/CCL3 coated Microplate (12 x 8 wells)", "number":"AB213916-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213916-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213916-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213916-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213916-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant rat MIP-1 alpha/CCL3 standard", "number":"AB213916-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213916-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213916-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213916-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.
Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.
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Product protocols

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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information Ccl3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Inflammopharmacology 33:3947-3967 PubMed40622464

2025

Crosstalk between ferroptosis and NLRP3, a possible therapeutic target in experimentally-induced rheumatoid arthritis: role of P2Y12R inhibition in modulating P53/SLC7A11/ALOX15 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma S Eltyar,Dalia M El-Tanbouly,Hala F Zaki,Rehab M El-Sayed

Toxins 12: PubMed33238460

2020

Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Jakšić,Ida Ćurtović,Domagoj Kifer,Dubravka Rašić,Nevenka Kopjar,Vedran Micek,Maja Peraica,Maja Šegvić Klarić
View all publications
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