AB242242

Rat MIP-1 alpha/CCL3 ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Rat MIP-1 alpha/CCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat MIP-1 alpha/CCL3 with a sensitivity of 1.96 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, Small-inducible cytokine A3, MIP-1-alpha

3 Images

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

sELISA

Unknown

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

Interpolated concentrations of spiked CCL3 in rat serum and plasma samples. The concentrations of CCL3 were measured in duplicates, interpolated from the CCL3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) 50% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Unknown

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

Interpolated concentrations of native CCL3 in stimulated and unstimulated rat PBMC supernatant samples. The concentrations of CCL3 were measured in duplicates, interpolated from the CCL3 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated rat PBMC supernatant 5% and unstimulated rat PBMC supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL3 concentration was determined to be 5,805 pg/mL in stimulated rat PBMC supernatant and was undetectable in unstimulated rat PBMC supernatant.

sELISA

Unknown

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

Example of rat CCL3 standard curve in Sample Diluent NS. The CCL3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.96 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat MIP-1 alpha/CCL3 ELISA Kit (ab242242) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP-1 alpha/CCL3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat MIP-1 alpha/CCL3 with 1.96 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 93 %", "average": "= 91" }, { "sample": "Serum", "range": "82 - 85 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "72 - 78 %", "average": "= 75" }, { "sample": "Heparin Plasma", "range": "81 - 89 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "77 - 78 %", "average": "= 78" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242242-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242242-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242242-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242242-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242242-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242242-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242242-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB242242-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat MIP-1 alpha/CCL3 Lyophilized Recombinant Protein", "number":"AB242242-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat MIP-1 alpha/CCL3 Capture Antibody (Lyophilized)", "number":"AB242242-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat MIP-1 alpha/CCL3 Detector Antibody", "number":"AB242242-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.

Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.

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Product protocols

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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

See full target information Ccl3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:770 PubMed39143617

2024

Sciatic nerve stimulation alleviates neuropathic pain and associated neuroinflammation in the dorsal root ganglia in a rodent model.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-En Wong,Wentai Liu,Chi-Chen Huang,Po-Hsuan Lee,Han-Wei Huang,Yu Chang,Hsin-Tien Lo,Hui-Fang Chen,Li-Chieh Kuo,Jung-Shun Lee

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat MIP-1 alpha/CCL3 ELISA Kit

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

Sandwich ELISA - Rat MIP-1 alpha/CCL3 ELISA Kit (AB242242)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise