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AB171336

Rat MIP-1 beta ELISA Kit (CCL4)

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(1 Publication)

Rat MIP-1 beta ELISA Kit (CCL4) is a Sandwich (quantitative) ELISA for the measurement of Rat MIP-1 beta (CCL4) in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

Mip1b, Scya4, Ccl4, C-C motif chemokine 4, Macrophage inflammatory protein 1-beta, Small-inducible cytokine A4, MIP-1-beta

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Sandwich ELISA - Rat MIP-1 beta ELISA Kit (CCL4) (AB171336)
  • sELISA

Supplier Data

Sandwich ELISA - Rat MIP-1 beta ELISA Kit (CCL4) (AB171336)

Representative Standard Curve using ab171336.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's rat MIP-1 beta (CCL4) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat MIP-1 beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

A MIP-1 beta specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MIP-1 beta is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat MIP-1 beta amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "148 pg/mL", "standardDeviation": "12.13", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "453 pg/mL", "standardDeviation": "24.31", "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "31 pg/mL", "standardDeviation": "1.67", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "31 pg/mL", "standardDeviation": "1.42", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "144 pg/mL", "standardDeviation": "9.21", "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "419 pg/mL", "standardDeviation": "24.3", "coefficientOfVariability": "= 5.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171336-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171336-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-rat MIP-1 beta antibody", "number":"AB171336-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-rat MIP-1 beta Antibody Microplate (12 x 8 wells)", "number":"AB171336-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171336-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171336-CMP10", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171336-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171336-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant rat MIP-1 beta standard", "number":"AB171336-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171336-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171336-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171336-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
Biological function summary

CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.

Pathways

CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.

CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
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Product protocols

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Target data

Monokine with inflammatory and chemokinetic properties.
See full target information Ccl4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 14:2925-2930 PubMed28912850

2017

Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Danyan Bi,Danqing Bi,Ming Zhong,Hong Zhang,Song Jin,Sha Ma,Huayou Luo
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