Rat MIP2 ELISA Kit (CXCL2)
- Recombinant
- SimpleStep
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Rat MIP2 ELISA Kit (CXCL2) is a single-wash 90-min Simplestep used to quantify Rat MIP2 (CXCL2) with a sensitivity of 0.37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Cinc3, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2, CINC-3, MIP2
- sELISA
Supplier Data
Sandwich ELISA - Rat MIP2 ELISA Kit (CXCL2) (AB282308)
Rat MIP2 competitor curve comparison.
Standard Curve comparison between Rat MIP2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Rat MIP2 ELISA Kit (CXCL2) (AB282308)
Interpolated concentrations of native MIP2 in rat serum, lung supernatant treated with or without 10 µg/mL Con A for 3 days, and heart supernatant samples.
The concentrations of MIP2 were measured in duplicates, interpolated from the MIP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, lung supernatant treated with Con A 0.5%, lung supernatant mock treated 1.25%, heart supernatant 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP2 concentration was determined to be 104 pg/mL in neat serum, 35,588 pg/mL in neat Con A treated lung supernatant, 17,197 in neat mock treated lung supernatant and 10,566 pg/mL in neat heart supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Rat MIP2 ELISA Kit (CXCL2) (AB282308)
Example of rat MIP2 standard curve in Sample Diluent NS.
The MIP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Rat MIP2 ELISA kit (ab282308) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat MIP2 protein in rat serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat MIP2 with 0.37 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes. Mature chain of rat MIP2 is 79% identical to rat C-X-C Motif Chemokine 3, and 74% identical to rat Growth-Regulated Alpha Protein. Rat MIP2 is 88% identical to mouse MIP2.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
Product protocols
- Visit the General protocols
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Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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