AB270216

Rat MMP3 ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Rat MMP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat MMP3 with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Stromelysin-1, SL-1, Matrix metalloproteinase-3, PTR1 protein, Transin-1, MMP-3, Mmp3

3 Images

ELISA

Supplier Data

ELISA - Rat MMP3 ELISA Kit (AB270216)

sELISA

Supplier Data

Sandwich ELISA - Rat MMP3 ELISA Kit (AB270216)

Representative Standard Curve using ab270216

sELISA

Supplier Data

Sandwich ELISA - Rat MMP3 ELISA Kit (AB270216)

Interpolated concentrations of native MMP3 in rat serum and plasma samples.

The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 66.05 ng/mL in serum, 73.89 ng/mL in plasma (citrate) and 56.23 ng/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 42 pg/mL

Range

273 - 17500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat MMP3 ELISA Kit ab270216 is a rapid single-wash 90-min Sandwich ELISA to measure Rat MMP3 in serum. This SimpleStep sensitivity is 42 pg/mL.

How the assay works

Rat MMP3 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat MMP3 ELISA Kit ab270216 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP3 antibody [EPR22606-163] - BSA and Azide free (Capture) ab272299
- Anti-MMP3 antibody [EPR22606-130] - BSA and Azide free (Detector) ab272299

Matrix metalloprotease-3 (MMP3), also known as Stromelysin-1, is a 54 kD secreted protease involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. It also has known roles in disease processes, such as arthritis and cancer metastasis. MMP3 functions by degrading fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. These actions are linked to activation of procollagenase to further help in the remodeling of the extracellular matrix environment. Rat MMP3 shares 76.2%, 90.2%, and 75.1% sequence homology with human, mouse, and cow, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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Recovery

[ { "sample": "Serum", "range": "86 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "89 - 97 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "88 - 94 %", "average": "= 90" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.

Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.

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Product protocols

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Target data

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.. Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities.

See full target information Mmp3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta biomaterialia 174:104-115 PubMed38081445

2023

In vitro dose-dependent effects of matrix metalloproteinases on ECM hydrogel biodegradation.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Didwischus,Arun Guduru,Stephen F Badylak,Michel Modo

Scientific reports 13:13034 PubMed37563154

2023

Investigation of the phytochemical composition, antioxidant, antibacterial, anti-osteoarthritis, and wound healing activities of selected vegetable waste.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Salem,Osama G Mohamed,Esraa M Mosalam,Aya Ibrahim Elberri,Hend Mohamed Abdel-Bar,Mariam Hassan,Ahmed A Al-Karmalawy,Ashootosh Tripathi,Shahira M Ezzat,Hend E Abo Mansour

Cannabis and cannabinoid research 9:147-159 PubMed36342775

2022

Application of Oil-in-Water Cannabidiol Emulsion for the Treatment of Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Petr Jelínek,Jaroslava Roušarová,Pavel Ryšánek,Martina Ježková,Tereza Havlůjová,Jiří Pozniak,Petr Kozlík,Tomáš Křížek,Tomáš Kučera,Martin Šíma,Ondřej Slanař,Miroslav Šoóš

International journal of molecular sciences 22: PubMed34638520

2021

Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Nian-Cih Huang,Tsorng-Shyang Yang,Prabhakar Busa,Ching-Ling Lin,Ya-Chieh Fang,Ing-Jung Chen,Chih-Shung Wong

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat MMP3 ELISA Kit

ELISA - Rat MMP3 ELISA Kit (AB270216)

Sandwich ELISA - Rat MMP3 ELISA Kit (AB270216)

Sandwich ELISA - Rat MMP3 ELISA Kit (AB270216)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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