Rat MMP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat MMP3 with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Scientific reports 13:130342023Investigation of the phytochemical composition, antioxidant, antibacterial, anti-osteoarthritis, and wound healing activities of selected vegetable waste.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed A Salem,Osama G Mohamed,Esraa M Mosalam,Aya Ibrahim Elberri,Hend Mohamed Abdel-Bar,Mariam Hassan,Ahmed A Al-Karmalawy,Ashootosh Tripathi,Shahira M Ezzat,Hend E Abo MansourPubMed 37563154
- International journal of molecular sciences 22:2021Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNian-Cih Huang,Tsorng-Shyang Yang,Prabhakar Busa,Ching-Ling Lin,Ya-Chieh Fang,Ing-Jung Chen,Chih-Shung WongPubMed 34638520
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 273 - 17500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 42 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities.
Alternative names
Stromelysin-1, SL-1, Matrix metalloproteinase-3, PTR1 protein, Transin-1, MMP-3, Mmp3
What's included?
Recommended products
Rat MMP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat MMP3 with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 273 - 17500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 42 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 86 - 93 % |
Sample type Citrate plasma | Average % = 93 | Range 89 - 97 % |
Sample type Heparin Plasma | Average % = 90 | Range 88 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat MMP3 ELISA Kit ab270216 is a rapid single-wash 90-min Sandwich ELISA to measure Rat MMP3 in serum. This SimpleStep sensitivity is 42 pg/mL.
How the assay works
Rat MMP3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Rat MMP3 ELISA Kit ab270216 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-MMP3 antibody [EPR22606-163] - BSA and Azide free (Capture) Anti-MMP3 antibody [EPR22606-130] - BSA and Azide free (Detector) ab272299
- Anti-MMP3 antibody [EPR22606-130] - BSA and Azide free (Detector) Anti-MMP3 antibody [EPR22606-130] - BSA and Azide free (Detector) ab272299
Matrix metalloprotease-3 (MMP3), also known as Stromelysin-1, is a 54 kD secreted protease involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. It also has known roles in disease processes, such as arthritis and cancer metastasis. MMP3 functions by degrading fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. These actions are linked to activation of procollagenase to further help in the remodeling of the extracellular matrix environment. Rat MMP3 shares 76.2%, 90.2%, and 75.1% sequence homology with human, mouse, and cow, respectively.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
- Biological function summary
MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.
- Pathways
MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.
- Associated diseases and disorders
MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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2 product images
ELISA - Rat MMP3 ELISA Kit (ab270216)
Sandwich ELISA - Rat MMP3 ELISA Kit (ab270216)
Interpolated concentrations of native MMP3 in rat serum and plasma samples.
The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 66.05 ng/mL in serum, 73.89 ng/mL in plasma (citrate) and 56.23 ng/mL in plasma (heparin).
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