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AB100779

Rat MMP8 ELISA Kit

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(1 Publication)

Rat MMP8 ELISA Kit is a Sandwich ELISA for the measurement of Rat MMP8 in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Neutrophil collagenase, Matrix metalloproteinase-8, MMP-8, Mmp8

2 Images
Sandwich ELISA - Rat MMP8 ELISA Kit (AB100779)
  • sELISA

Unknown

Sandwich ELISA - Rat MMP8 ELISA Kit (AB100779)

Representative standard curve using ab100779

Sandwich ELISA - Rat MMP8 ELISA Kit (AB100779)
  • sELISA

Unknown

Sandwich ELISA - Rat MMP8 ELISA Kit (AB100779)

Typical data B.

Representative standard curve using ab100779

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 50 pg/mL

Range

0.05 - 40 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.

This assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 108 %", "average": "= 95.42" }, { "sample": "Serum", "range": "99 - 118 %", "average": "= 109.78" }, { "sample": "Plasma", "range": "98 - 118 %", "average": "= 107.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100779-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100779-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100779-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"160X HRP-Streptavidin Concentrate", "number":"AB100779-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100779-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MMP8 Microplate (12 x 8 wells)", "number":"AB100779-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat MMP8 Standard (lyophilized)", "number":"AB100779-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat MMP8", "number":"AB100779-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100779-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100779-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.
Biological function summary

MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.

Pathways

MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.

MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.
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Product protocols

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Target data

Can degrade fibrillar type I, II, and III collagens.
See full target information Mmp8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American So 22:786-96 PubMed24402185

2014

A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Monaghan,Shane Browne,Katja Schenke-Layland,Abhay Pandit
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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