AB285308

Rat Myeloperoxidase ELISA Kit

Name: Rat Myeloperoxidase ELISA Kit (ab285308) | Abcam
Brand: Abcam Limited
SKU: AB285308

Be the first to review this product! Submit a review

(1 Publication)

Rat Myeloperoxidase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Myeloperoxidase in Rat in Biofluids, Cell/Tissue Extracts samples.

1 Images

Sandwich ELISA - Rat Myeloperoxidase ELISA Kit (AB285308)

sELISA

Supplier Data

Sandwich ELISA - Rat Myeloperoxidase ELISA Kit (AB285308)

Rat Myeloperoxidase ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 0.469 ng/mL

Range

0.78 - 50 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Myeloperoxidase (MPO) is a peroxidase enzyme most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. MPO contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H₂O₂) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). MPO also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

This Rat Myeloperoxidase ELISA kit (ab285308, E4581) is a sandwich ELISA assay for the quantitative measurement of MPO in Rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of MPO captured from the samples.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]

style

left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285308-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285308-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285308-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285308-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285308-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285308-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285308-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample / Standard Dilution Buffer", "number":"AB285308-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285308-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285308-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285308-CMP01", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285308-CMP03", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

style

left-column

Product protocols

style

left-column

Target data

See full target information Mpo

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuromolecular medicine 25:301-311 PubMed36749430

2023

The Role of PGK1 in Promoting Ischemia/Reperfusion Injury-Induced Microglial M1 Polarization and Inflammation by Regulating Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cao,Zhengzhe Feng,Deyuan Zhu,Suya Li,Meng Du,Shifei Ye,Dayong Qi,Peng Li,Yan Chen,Yibin Fang

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Myeloperoxidase ELISA Kit

Sandwich ELISA - Rat Myeloperoxidase ELISA Kit (AB285308)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise