Rat Myoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Myoglobin in Rat in Plasma, Serum samples.
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- Function (Oxford, England) 4:zqad0222023Post-injury Inhibition of Endothelin-1 Dependent Renal Vasoregulation Mitigates Rhabdomyolysis-Induced Acute Kidney Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremiah M Afolabi,Praghalathan Kanthakumar,Jada D Williams,Ravi Kumar,Hitesh Soni,Adebowale AdebiyiPubMed 37342410
- Chinese journal of traumatology = Zhonghua chuang shang za zhi 21:88-952018Response of macrophages in rat skeletal muscle after eccentric exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQun Zuo,Shu-Chen Wang,Xin-Kai Yu,Wei-Wei ChaoPubMed 29550244
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 6.25 - 200 ng/mL
- Sensitivity
- = 3.365 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:3118370). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (By similarity).
Alternative names
Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, Mb
What's included?
Recommended products
Rat Myoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Myoglobin in Rat in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 6.25 - 200 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 3.365 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % > 85 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Myoglobin Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin levels in rat serum and plasma.
In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myoglobin also known as MB is a small globular protein with a molecular weight of approximately 17 kDa. It functions as an oxygen-binding protein and is expressed mainly in cardiac and skeletal muscle tissue where it facilitates oxygen storage and transport. The myoglobin protein plays an important role in maintaining the oxygen supply needed during muscular contraction and intense physical activity.
- Biological function summary
Myoglobin in muscle cells acts to store oxygen which provides a rapid release when required during muscle contraction. Myoglobin serves as a monomer and does not form part of a complex. Its structure allows it to temporarily store and relay oxygen where it is most required enhancing the often abrupt demands of muscles for oxygen. The ability of myoglobin to bind oxygen and release it under hypoxic conditions is central to its biological role in vertebrates.
- Pathways
The function of myoglobin in aerobic respiration in muscles involves its participation in the oxygen transport pathway. This protein closely interacts with hemoglobin to mobilize oxygen effectively to mitochondria during muscle contraction. Unlike hemoglobin myoglobin has a hyperbolic oxygen dissociation curve which allows it to provide oxygen at lower partial pressures contributing significantly to the efficient metabolism during hypoxia or intense muscular exertion.
- Associated diseases and disorders
Myoglobin plays a significant role in conditions such as rhabdomyolysis and myocardial infarction. Rhabdomyolysis a syndrome caused by muscle injury results in the release of myoglobin into the bloodstream. Myoglobin detection kits including myoglobin ELISA are essential tools for the diagnosis of these conditions. Moreover its rapid increase in plasma levels after heart muscle damage enables its use as an early marker for myocardial infarction. Myoglobin's interaction with proteins like creatine kinase provides valuable information on muscle damage and cardiac events.
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1 product image
Sandwich ELISA - Rat Myoglobin ELISA Kit (ab157739)
Representative standard curve using ab157739 Myoglobin Rat ELISA Kit.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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