Rat Myoglobin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat Myoglobin in Tissue Extracts, Cell culture media, Cell culture supernatant, Serum, EDTA Plasma samples.
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- The Journal of international medical research 49:3000605219933152021Atractylenolide I alleviates ischemia/reperfusion injury by preserving mitochondrial function and inhibiting caspase-3 activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaiqin Sun,Xuesong Zhang,Fei Yu,Chen Liu,Fangbin Hu,Li Liu,Jing Chen,Jue WangPubMed 33641489
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Cell culture media, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
0.47 - 30 ng/mL
- Assay time
1h 30m
- Sensitivity
= 0.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:3118370). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (By similarity).
Alternative names
Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, Mb
What's included?
Recommended products
Rat Myoglobin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat Myoglobin in Tissue Extracts, Cell culture media, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Cell culture media, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
0.47 - 30 ng/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.1 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97 | Range 91 - 106 % |
Sample type Serum | Average % = 89 | Range 86 - 91 % |
Sample type EDTA Plasma | Average % = 106 | Range 95 - 119 % |
Sample type Tissue Extracts | Average % = 109 | Range 103 - 117 % |
Sample type Cell culture media | Average % = 87 | Range 82 - 90 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Rat Myoglobin ELISA Kit (ab260068) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in edta plasma, serum, cell culture media, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Myoglobin with 0.1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Myoglobin, is a 18.4 kDa heme protein, found in both cardiac and skeletal muscle that functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours. Rat Myoglobin shares 83%, 93%, 77%, and 84% sequence homology with human, mouse, bovine, and monkey respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myoglobin also known as MB is a small globular protein with a molecular weight of approximately 17 kDa. It functions as an oxygen-binding protein and is expressed mainly in cardiac and skeletal muscle tissue where it facilitates oxygen storage and transport. The myoglobin protein plays an important role in maintaining the oxygen supply needed during muscular contraction and intense physical activity.
- Biological function summary
Myoglobin in muscle cells acts to store oxygen which provides a rapid release when required during muscle contraction. Myoglobin serves as a monomer and does not form part of a complex. Its structure allows it to temporarily store and relay oxygen where it is most required enhancing the often abrupt demands of muscles for oxygen. The ability of myoglobin to bind oxygen and release it under hypoxic conditions is central to its biological role in vertebrates.
- Pathways
The function of myoglobin in aerobic respiration in muscles involves its participation in the oxygen transport pathway. This protein closely interacts with hemoglobin to mobilize oxygen effectively to mitochondria during muscle contraction. Unlike hemoglobin myoglobin has a hyperbolic oxygen dissociation curve which allows it to provide oxygen at lower partial pressures contributing significantly to the efficient metabolism during hypoxia or intense muscular exertion.
- Associated diseases and disorders
Myoglobin plays a significant role in conditions such as rhabdomyolysis and myocardial infarction. Rhabdomyolysis a syndrome caused by muscle injury results in the release of myoglobin into the bloodstream. Myoglobin detection kits including myoglobin ELISA are essential tools for the diagnosis of these conditions. Moreover its rapid increase in plasma levels after heart muscle damage enables its use as an early marker for myocardial infarction. Myoglobin's interaction with proteins like creatine kinase provides valuable information on muscle damage and cardiac events.
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4 product images
Sandwich ELISA - Rat Myoglobin ELISA Kit (ab260068)
Example of rat Myoglobin standard curve in Sample Diluent NS.
The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Myoglobin ELISA Kit (ab260068)
Interpolated concentrations of native Myoglobin in rat serum and plasma (EDTA) samples.
The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 10.60 ng/mL in serum and 16.61 ng/mL in plasma (EDTA).
Sandwich ELISA - Rat Myoglobin ELISA Kit (ab260068)
Interpolated concentrations of native Myoglobin in rat heart tissue culture supernatant samples.
The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: heart tissue culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 1927.15 ng/mL in heart tissue culture supernatant.
Sandwich ELISA - Rat Myoglobin ELISA Kit (ab260068)
Interpolated concentrations of native Myoglobin in rat heart extract and skeletal muscle extract based on a 50 ng/mL extract load.
The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 5.28 ng/mL in heart extract and 5.87 ng/mL in skeletal muscle extract.
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