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AB260068

Rat Myoglobin ELISA Kit

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(1 Publication)

Rat Myoglobin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Myoglobin with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, Mb

4 Images
Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)

Interpolated concentrations of native Myoglobin in rat heart extract and skeletal muscle extract based on a 50 ng/mL extract load.

The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 5.28 ng/mL in heart extract and 5.87 ng/mL in skeletal muscle extract.

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)

Example of rat Myoglobin standard curve in Sample Diluent NS.

The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)

Interpolated concentrations of native Myoglobin in rat heart tissue culture supernatant samples.

The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : heart tissue culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 1927.15 ng/mL in heart tissue culture supernatant.

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Myoglobin ELISA Kit (AB260068)

Interpolated concentrations of native Myoglobin in rat serum and plasma (EDTA) samples.

The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 10.60 ng/mL in serum and 16.61 ng/mL in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell culture media, Tissue supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.47 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat Myoglobin ELISA Kit (ab260068) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in edta plasma, serum, cell culture media, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Myoglobin with 0.1 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Myoglobin, is a 18.4 kDa heme protein, found in both cardiac and skeletal muscle that functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours. Rat Myoglobin shares 83%, 93%, 77%, and 84% sequence homology with human, mouse, bovine, and monkey respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 106 %", "average": "= 97" }, { "sample": "Serum", "range": "86 - 91 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "95 - 119 %", "average": "= 106" }, { "sample": "Tissue Extracts", "range": "103 - 117 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "82 - 90 %", "average": "= 87" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myoglobin also known as MB is a small globular protein with a molecular weight of approximately 17 kDa. It functions as an oxygen-binding protein and is expressed mainly in cardiac and skeletal muscle tissue where it facilitates oxygen storage and transport. The myoglobin protein plays an important role in maintaining the oxygen supply needed during muscular contraction and intense physical activity.
Biological function summary

Myoglobin in muscle cells acts to store oxygen which provides a rapid release when required during muscle contraction. Myoglobin serves as a monomer and does not form part of a complex. Its structure allows it to temporarily store and relay oxygen where it is most required enhancing the often abrupt demands of muscles for oxygen. The ability of myoglobin to bind oxygen and release it under hypoxic conditions is central to its biological role in vertebrates.

Pathways

The function of myoglobin in aerobic respiration in muscles involves its participation in the oxygen transport pathway. This protein closely interacts with hemoglobin to mobilize oxygen effectively to mitochondria during muscle contraction. Unlike hemoglobin myoglobin has a hyperbolic oxygen dissociation curve which allows it to provide oxygen at lower partial pressures contributing significantly to the efficient metabolism during hypoxia or intense muscular exertion.

Myoglobin plays a significant role in conditions such as rhabdomyolysis and myocardial infarction. Rhabdomyolysis a syndrome caused by muscle injury results in the release of myoglobin into the bloodstream. Myoglobin detection kits including myoglobin ELISA are essential tools for the diagnosis of these conditions. Moreover its rapid increase in plasma levels after heart muscle damage enables its use as an early marker for myocardial infarction. Myoglobin's interaction with proteins like creatine kinase provides valuable information on muscle damage and cardiac events.
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Product protocols

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Target data

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed : 3118370). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (By similarity).
See full target information Mb
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of international medical research 49:300060521993315 PubMed33641489

2021

Atractylenolide I alleviates ischemia/reperfusion injury by preserving mitochondrial function and inhibiting caspase-3 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Caiqin Sun,Xuesong Zhang,Fei Yu,Chen Liu,Fangbin Hu,Li Liu,Jing Chen,Jue Wang
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Product promise

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