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AB282300

Rat NRG1 ELISA Kit

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Rat NRG1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat NRG1 with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ndf, Nrg1, Pro-NRG1

2 Images
Sandwich ELISA - Rat NRG1 ELISA Kit (AB282300)
  • sELISA

Supplier Data

Sandwich ELISA - Rat NRG1 ELISA Kit (AB282300)

Example of rat NRG1 standard curve in Sample Diluent NS.

The NRG1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat NRG1 ELISA Kit (AB282300)
  • sELISA

Supplier Data

Sandwich ELISA - Rat NRG1 ELISA Kit (AB282300)

Interpolated concentrations of spike NRG1 in rat serum and plasma samples.

The concentrations of NRG1 were measured in duplicates, interpolated from the NRG1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 36 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat NRG1 ELISA kit (ab282300) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat NRG1 protein in rat serum, plasma, and cell culture superntant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat NRG1 with 36.0 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

NRG1 is a type of signal protein encoded by the NRG1 gene that is one of the four proteins in the neuregulin family. NRG1 is known to recognize the EGFR family as well to activate the ERBB family for cell differentiation, proliferation, migration, and development in neurons and oligodendrocytes. Studies show when injecting NRG1 gene in Schwann cells can significantly increase the effect on the repair of spinal cord injury and provide protection of neurons from apoptosis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.28" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Serum", "range": "118 - 120 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "114 - 118 %", "average": "= 116" }, { "sample": "Cell culture media", "range": "119 - 121 %", "average": "= 120" }, { "sample": "Heparin Plasma", "range": "80 - 80 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "100 - 111 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuregulin 1 (NRG1) also known as heregulin beta 1 is a member of the neuregulin protein family. It is a signaling protein with a molecular mass that typically ranges around 44 kDa. Researchers have found NRG1 expressed in various tissues including the heart brain and certain epithelial cells. It plays important roles in cell-cell communication by binding to ErbB family receptors particularly ErbB3 and ErbB4 which trigger downstream signaling processes.
Biological function summary

NRG1 is involved in several cellular activities such as cell growth survival and differentiation. It is not part of a complex but acts directly through its interactions with receptor tyrosine kinases in the ErbB receptor family. NRG1 influences cardiac development and neural function by mediating these cellular processes promoting the development and maintenance of neural and cardiac tissues.

Pathways

NRG1 is involved in the PI3K-Akt and MAPK/ERK signaling pathways. These pathways are critical for cellular growth differentiation and survival supporting the functions of NRG1 in various tissues. The interaction with ErbB2 and ErbB3 receptors is especially significant for these pathways as they help regulate the cellular responses initiated by NRG1 signaling.

NRG1 plays an impactful role in the development of certain types of cancer and schizophrenia. Dysregulation or mutations in the NRG1 gene can lead to altered signaling pathways which are connected to the pathogenesis of these conditions. In cancer the interaction with ErbB2 has been under investigation as it may promote tumorigenesis. In the context of schizophrenia NRG1's effects on neural development and neurotransmission are linked with the disorder's symptoms.
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Product protocols

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Target data

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Binds to ERBB4 and ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV : ITGB3 or ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling (By similarity). Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1, and ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (PubMed : 17250808).
See full target information Nrg1
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websiteProtocolBooklet
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