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AB205076

Rat Osteopontin ELISA kit

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(1 Publication)

Rat Osteopontin ELISA kit is a Sandwich ELISA for the measurement of Rat Osteopontin in Rat in Biofluids samples.

View Alternative Names

2b7, Spp-1, Spp1, Osteopontin, Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1

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Sandwich ELISA - Rat Osteopontin ELISA kit (AB205076)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Osteopontin ELISA kit (AB205076)

Representative standard curve using ab205076 Rat Osteopontin ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.02 ng/mL

Range

0.06 - 4 ng/mL

Assay time

3h 20m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB63856

Anti-Osteopontin antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody (AB63856)

  • 0
  • 1
Primary Antibodies

AB75285

Anti-OPN3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-OPN3 antibody (AB75285)

  • 0
  • 0
Primary Antibodies

AB133612

Anti-Osteocalcin antibody [EPR3690]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

  • 0
  • 0
Primary Antibodies

AB192256

Anti-RUNX2 antibody [EPR14334]

Recombinant|Advanced Validation|RabMAb|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR14334] (AB192256)

  • 5
  • 9
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Osteopontin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Osteopontin protein in rat plasma, serum and urine.

In this assay the Osteopontin present in samples reacts with the anti-Osteopontin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Osteopontin, is added and complexes are formed. Following a wash step, horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Osteopontin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Osteopontin in the test sample. The quantity of Osteopontin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205076-1x96Tests", "assetComponentDetails": [ { "size":"1 x 60 mL", "name":"1X Diluent Concentrate", "number":"AB205076-CMP07", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X Detection Antibody Conjugate", "number":"AB205076-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Osteopotin Calibrator (Lyophilized)", "number":"AB205076-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rat Osteopontin ELISA Microplate", "number":"AB205076-CMP01", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-Streptavidin", "number":"AB205076-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205076-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205076-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205076-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
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Product protocols

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
See full target information Spp1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 44:760-770 PubMed28394420

2017

Tiron ameliorates high glucose-induced cardiac myocyte apoptosis by PKCδ-dependent inhibition of osteopontin.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Jiang,Deling Zhang,Hong Qiu,Xianqi Yi,Yemin Zhang,Yingkang Cao,Bo Zhao,Zhongyuan Xia,Changhua Wang
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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