AB254514

Rat Osteopontin ELISA Kit (OPN)

Recombinant
SimpleStep
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(1 Publication)

Rat Osteopontin ELISA Kit (OPN) is a single-wash 90-min Simplestep used to quantify Rat Osteopontin (OPN) with a sensitivity of 16.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

2b7, Spp-1, Spp1, Osteopontin, Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1

4 Images

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

sELISA

Supplier Data

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Representative image of Rat Osteopontin ELISA Kit (OPN) (ab254514)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Rat Osteopontin (OPN) Capture Antibody

- 10X Rat Osteopontin (OPN) Detector Antibody

- Rat Osteopontin (OPN) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Interpolated concentrations of native Osteopontin in rat lung and spleen tissue culture supernatant samples.

The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : rat lung tissue supernatant 6.25% and rat spleen tissue supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 24.49 ng/mL in neat rat lung tissue supernatant and 22.77 ng/mL in neat rat spleen tissue supernatant.

sELISA

Supplier Data

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Interpolated concentrations of native Osteopontin in rat serum and plasma samples. The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves.

The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, plasma (heparin) 12.5% and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 16.57 ng/mL in neat serum, 14.99 ng/mL in neat plasma (citrate), 21.52 ng/mL in neat plasma (heparin), and 17.21 ng/mL in neat plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Example of rat Osteopontin standard curve in 1X Cell Extraction Buffer PTR.

The Osteopontin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 16.8 pg/mL

Range

50 - 3200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Osteopontin ELISA Kit (OPN) (ab254514) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteopontin (OPN) protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Osteopontin (OPN) with 16.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Osteopontin, known also as Secreted Phosphoprotein 1, is a secreted adhesive glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein family (SIBLING) and is expressed mainly in bone, teeth, kidney, epithelial lining tissues, activated T-cells and macrophages and found in serum and plasma. It is processed by matrix metalloproteases 3 and 4 as well as thrombin, which generate two functional fragments: an N terminal (RGD) fragment that binds to integrin receptors and a C terminal fragment (containing heparin binding sites) that interacts with CD44. Osteopontin can be present as a cytokine-like soluble protein or an immobilized extracellular matrix protein. Osteopontin is important in multiple physiologic and pathologic processes including survival, adhesion, migration, angiogenesis, apoptosis, inflammation, macrophage migration, B cell proliferation, Ig production, wound healing and tumor metastasis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Serum", "range": "98 - 101 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "90 - 104 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "101 - 119 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "101 - 115 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "103 - 109 %", "average": "= 106" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254514-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254514-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254514-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB254514-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254514-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254514-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB254514-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Osteopontin (OPN) Capture Antibody", "number":"AB254514-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB254514-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Osteopontin (OPN) Lyophilized Recombinant Protein", "number":"AB254514-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Rat Osteopontin (OPN) Detector Antibody", "number":"AB254514-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB254514-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254514-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.

Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.

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Product protocols

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

See full target information Spp1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 527:111171 PubMed33577975

2021

Estrogen promotes lncRNA H19 expression to regulate osteogenic differentiation of BMSCs and reduce osteoporosis via miR-532-3p/SIRT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Hongxia Jiang,Yang Li,Xiaojie Zhao,Hui Ding

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rat Osteopontin ELISA Kit (OPN)

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Sandwich ELISA - Rat Osteopontin ELISA Kit (OPN) (AB254514)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise