Rat Osteoprotegerin ELISA Kit (OPG)
- Recombinant
- SimpleStep
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(3 Publications)
Rat Osteoprotegerin ELISA Kit (OPG) is a single-wash 90-min Simplestep used to quantify Rat Osteoprotegerin (OPG) with a sensitivity of 30 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Opg, Tnfrsf11b, Tumor necrosis factor receptor superfamily member 11B, Osteoprotegerin
- sELISA
Supplier Data
Sandwich ELISA - Rat Osteoprotegerin ELISA Kit (OPG) (AB255723)
Interpolated concentrations of native Osteoprotegerin in rat serum and plasma samples.
The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (heparin) 10% and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteoprotegerin concentration was determined to be 2216.74 pg/mL in serum, 1852.15 pg/mL in plasma (citrate) and 2107.37 pg/mL in plasma (heparin)and 1619.46 pg/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Rat Osteoprotegerin ELISA Kit (OPG) (AB255723)
Example of rat Osteoprotegerin standard curve in 1X Cell Extraction Buffer PTR.
The Osteoprotegerin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Product details
Rat Osteoprotegerin ELISA Kit (OPG) (ab255723) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin (OPG) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Osteoprotegerin (OPG) with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Osteoprotegerin is a cytokine in the TNF receptor superfamily found primarily cells derived from osteoblasts. Its primary role is in bone metabolism where it inhibits bone resorption. Reduced osteoprotegerin signaling leads is one of the key mechanisms for the development of osteoporosis and is a hallmark of many metastatic bone tumors.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
Product protocols
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Publications (3)
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Scientific reports 15:20701 PubMed40596392
2025
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Frontiers in cell and developmental biology 9:762335 PubMed34790669
2021
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Medical science monitor : international medical jo 26:e918541 PubMed31911574
2020
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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