Rat Osteoprotegerin ELISA Kit (OPG) is a single-wash 90-min Simplestep used to quantify Rat Osteoprotegerin (OPG) with a sensitivity of 30 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in cell and developmental biology 9:7623352021Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanping Gong,Yang Wang,Yiqing Zhang,Liangchen Wang,Lijuan Wan,Yuan Zu,Chunlin Li,Xin Wang,Zhong-Kai CuiPubMed 34790669
- Medical science monitor : international medical jo 26:e9185412020N-myc Downstream-Regulated Gene 2 (NDRG2) Promotes Bone Morphogenetic Protein 2 (BMP2)-Induced Osteoblastic Differentiation and Calcification by Janus Kinase 3 (JAK3)/Signal Transducer and Activator of Transcription 3 (STAT3) Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunYu Chen et. al.PubMed 31911574
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 30 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis (By similarity).
Alternative names
Opg, Tnfrsf11b, Tumor necrosis factor receptor superfamily member 11B, Osteoprotegerin
What's included?
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Rat Osteoprotegerin ELISA Kit (OPG) is a single-wash 90-min Simplestep used to quantify Rat Osteoprotegerin (OPG) with a sensitivity of 30 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 30 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 8.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105 | Range 96 - 118 % |
Sample type EDTA Plasma | Average % = 95 | Range 92 - 99 % |
Sample type Heparin Plasma | Average % = 94 | Range 90 - 106 % |
Sample type Citrate plasma | Average % = 101 | Range 93 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Osteoprotegerin ELISA Kit (OPG) (ab255723) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin (OPG) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Osteoprotegerin (OPG) with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Osteoprotegerin is a cytokine in the TNF receptor superfamily found primarily cells derived from osteoblasts. Its primary role is in bone metabolism where it inhibits bone resorption. Reduced osteoprotegerin signaling leads is one of the key mechanisms for the development of osteoporosis and is a hallmark of many metastatic bone tumors.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
- Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
- Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
- Associated diseases and disorders
OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
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2 product images
Sandwich ELISA - Rat Osteoprotegerin ELISA Kit (OPG) (ab255723)
Example of rat Osteoprotegerin standard curve in 1X Cell Extraction Buffer PTR.
The Osteoprotegerin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Osteoprotegerin ELISA Kit (OPG) (ab255723)
Interpolated concentrations of native Osteoprotegerin in rat serum and plasma samples.
The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (heparin) 10% and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteoprotegerin concentration was determined to be 2216.74 pg/mL in serum, 1852.15 pg/mL in plasma (citrate) and 2107.37 pg/mL in plasma (heparin)and 1619.46 pg/mL in plasma (EDTA).
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