AB255725

Rat OX40 ELISA Kit (TNFRSF4)

Recombinant
SimpleStep
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Rat OX40 ELISA Kit (TNFRSF4) is a single-wash 90-min Simplestep used to quantify Rat OX40 (TNFRSF4) with a sensitivity of 53 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD134, Ox40, Txgp1l, Tnfrsf4, Tumor necrosis factor receptor superfamily member 4, MRC OX40, OX40 antigen, OX40L receptor

2 Images

Sandwich ELISA - Rat OX40 ELISA Kit (TNFRSF4) (AB255725)

sELISA

Supplier Data

Sandwich ELISA - Rat OX40 ELISA Kit (TNFRSF4) (AB255725)

Example of rat OX40 standard curve in Sample Diluent NS.

The OX40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat OX40 ELISA Kit (TNFRSF4) (AB255725)

Interpolated concentrations of native OX40 in rat serum and plasma samples.

The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (edta) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 883.79 pg/mL in serum, 803.75 pg/mL in plasma (citrate), 882.79 pg/mL in plasma (heparin) and 642.09 pg/mL in plasma (edta).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 53 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat OX40 ELISA Kit (TNFRSF4) (ab255725) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OX40 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat OX40 with 53 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

OX40, also known as TNFRSF4 and/or CD134, is a constitutively expressed transmembrane receptor found on T-cells. OX40 serves as a secondary checkpoint for immunological responses through the binding of TNF receptor associated factor (TRAF) proteins. OX40 and its predominant ligand, OX40L, regulate adaptive T cell immune responses. Clinically, OX40 is the target of several immunotherapy antibody treatments to promote antitumor immunity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]

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Recovery

[ { "sample": "Serum", "range": "100 - 105 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "88 - 106 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "97 - 105 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

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Product protocols

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Target data

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity).

See full target information Tnfrsf4

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat OX40 ELISA Kit (TNFRSF4)

Sandwich ELISA - Rat OX40 ELISA Kit (TNFRSF4) (AB255725)

Sandwich ELISA - Rat OX40 ELISA Kit (TNFRSF4) (AB255725)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise