Rat PAI1 ELISA Kit (SERPINE1) is a Quantitative ELISA kit for the measurement of Rat PAI1 (SERPINE1) in Rat in Plasma, Serum samples.
Images
Publications
Filter by
- Stroke 50:3600-36082019Vepoloxamer Enhances Fibrinolysis of tPA (Tissue-Type Plasminogen Activator) on Acute Ischemic Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyang Wang et. al.PubMed 31587657
- Marine drugs 14:2016Undaria pinnatifida and Fucoxanthin Ameliorate Lipogenesis and Markers of Both Inflammation and Cardiovascular Dysfunction in an Animal Model of Diet-Induced Obesity.
- Scientific reports 6:304642016Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies.Applications:Unspecified applicationReactive species:RatHaitang Jiang,Xiaoli Li,Suzhen Chen,Na Lu,Yingying Yue,Jinfeng Liang,Zhijun Zhang,Yonggui YuanPubMed 27456456
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Quantitative
- Reactive species
- Rat
- Range
- 0.1 - 50 ng/mL
- Sensitivity
- = 0.079 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).
Alternative names
Pai1, Planh1, Serpine1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
What's included?
Recommended products
Rat PAI1 ELISA Kit (SERPINE1) is a Quantitative ELISA kit for the measurement of Rat PAI1 (SERPINE1) in Rat in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Quantitative
- Reactive species
- Rat
- Range
- 0.1 - 50 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.079 ng/mL
- General recovery
- 104%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 20 | mean 2.25 ng/mL | SD 0.134 | C.V. 5.97 |
Sample Spike | n 20 | mean 0.957 ng/mL | SD 0.088 | C.V. 9.24 |
Sample Spike | n 20 | mean 15.6 ng/mL | SD 1.01 | C.V. 6.48 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 10 | mean 1.4 ng/mL | SD 0.096 | C.V. 6.86 |
Sample Spike | n 10 | mean 0.926 ng/mL | SD 0.113 | C.V. 12.2 |
Sample Spike | n 10 | mean 9.17 ng/mL | SD 0.858 | C.V. 9.36 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Spike | Average % = 104 | Range 97 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's PAI1 (SERPINE1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active PAI1 (SERPINE1) in rat plasma and serum.
Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate and will not be detected. Unbound PAI1 samples are washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI1 present in the samples, is then bound by the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (ab198509)
Full range standard curve using ab198509 PAI1 (SERPINE1) Rat ELISA Kit.
Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (ab198509)
Linear range standard curve using ab198509 PAI1 (SERPINE1) Rat ELISA Kit.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com