AB201283

Rat PAI1 ELISA Kit (SERPINE1)

Recombinant
SimpleStep
What is this?

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(3 Publications)

Rat PAI1 ELISA Kit (SERPINE1) is a single-wash 90-min Simplestep used to quantify Rat PAI1 (SERPINE1) with a sensitivity of 159 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Pai1, Planh1, Serpine1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

3 Images

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

sELISA

Supplier Data

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Interpolated concentrations of PAI1 in rat serum.

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 5,897 pg/mL in mouse serum.

sELISA

Supplier Data

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Example of PAI1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Interpolated Concentration of PAI1 in rat serum, plasma-citrate, and urine.

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The Interpolated dilution factor corrected values are plotted (mean+/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Urine, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 159 pg/mL

Range

298 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat PAI1 ELISA Kit (SERPINE1) (ab201283) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat PAI1 (SERPINE1) with 159 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Rat Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Rat Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" } ]

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Recovery

[ { "sample": "Serum", "range": "94 - 105 %", "average": "= 100" }, { "sample": "Urine", "range": "69 - 88 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "84 - 87 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "98 - 102 %", "average": "= 99" }, { "sample": "serum free media", "range": "91 - 98 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).

See full target information Serpine1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG 40:e2023036 PubMed37712376

2023

Protective efficacy of pirfenidone in rats with pulmonary fibrosis induced by bleomycin.

Applications

Unspecified application

Species

Unspecified reactive species

Baris Demirkol,Sule Gul,Mustafa Cörtük,Neslihan Akanıl Fener,Eminegül Yavuzsan,Ramazan Eren,Kursad Nuri Baydili,Mustafa Baki Çekmen,Erdogan Cetinkaya

Bone & joint research 10:41-50 PubMed33448865

2021

Fibrinolysis as a target to enhance osteoporotic fracture healing by vibration therapy in a metaphyseal fracture model.

Applications

Unspecified application

Species

Unspecified reactive species

Ronald Man Yeung Wong,Victoria Man Huen Choy,Jie Li,Tsz Kiu Li,Yu Ning Chim,Meng Chen Michelle Li,Jack Chun Yiu Cheng,Kwok-Sui Leung,Simon Kwoon-Ho Chow,Wing Hoi Cheung

Journal of thrombosis and thrombolysis 45:77-87 PubMed29168147

2017

Generation and characterization of tissue-type plasminogen activator transgenic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Ito,Kengo Noguchi,Yoshiyuki Morishima,Kyoji Yamaguchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat PAI1 ELISA Kit (SERPINE1)

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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