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AB213906

Rat PDGF-AB ELISA Kit

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(2 Publications )

Rat PDGF-AB ELISA Kit is a Sandwich ELISA for the measurement of Rat PDGF-AB in Rat in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Rat PDGF-AB ELISA Kit (AB213906)
  • sELISA

Supplier Data

Sandwich ELISA - Rat PDGF-AB ELISA Kit (AB213906)

Rat PDGF-AB ELISA Kit (ab213906) Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

31.2 - 2000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Rat PDGF-AB Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213906) is designed for the quantitative measurement of Rat PDGF-AB in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-AB has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat PDGF-AB amount of sample captured in plate.

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF Bchain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.

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Precision & recovery information

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "275 pg/mL", "standardDeviation": "20.35", "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "723 pg/mL", "standardDeviation": "45.55", "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1249 pg/mL", "standardDeviation": "73.7", "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "246 pg/mL", "standardDeviation": "14.27", "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "632 pg/mL", "standardDeviation": "34.76", "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1068 pg/mL", "standardDeviation": "50.2", "coefficientOfVariability": "4.7" } ]

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information Pdgfb

Alternative Names

Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Pdgfb
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Theranostics 14:608-621 PubMed38169629

2024

Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Sandrine Parent,Ramana Vaka,Jennifer St Amant,Saad Kahn,Sophie Van Remortel,Christina Bi,David Courtman,Duncan John Stewart,Darryl Raymond Davis

JCI insight 8: PubMed37384420

2023

Prevention of atrial fibrillation after open-chest surgery with extracellular vesicle therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sandrine Parent,Ramana Vaka,Yousef Risha,Clarissa Ngo,Pushpinder Kanda,Stanley Nattel,Saad Khan,David Courtman,Duncan J Stewart,Darryl R Davis
View all publications
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Product promise

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