Rat Prolactin ELISA Kit is a Quantitative ELISA kit for the measurement of Rat Prolactin in Rat in Citrate plasma, EDTA Plasma, Heparin Plasma samples.
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- eNeuro 9:2022Milking It for All It's Worth: The Effects of Environmental Enrichment on Maternal Nurturance, Lactation Quality, and Offspring Social Behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHolly DeRosa,Salvatore G Caradonna,Hieu Tran,Jordan Marrocco,Amanda C KentnerPubMed 35995560
- Molecular medicine reports 23:2021MicroRNA‑29‑3p regulates the β‑catenin pathway by targeting IGF1 to inhibit the proliferation of prolactinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Xia,Songmei Li,Dianfei Ma,Wenyujie Guo,Hong Long,Weiping YinPubMed 33846792
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, EDTA Plasma, Heparin Plasma
- Assay type
- Quantitative
- Reactive species
- Rat
- Range
- 2 - 1000 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Decidual prolactin, GHA1, Growth hormone A1, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, Prolactin, Prolactin precursor
What's included?
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Rat Prolactin ELISA Kit is a Quantitative ELISA kit for the measurement of Rat Prolactin in Rat in Citrate plasma, EDTA Plasma, Heparin Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, EDTA Plasma, Heparin Plasma
- Assay type
- Quantitative
- Reactive species
- Rat
- Range
- 2 - 1000 ng/mL
- Assay Platform
- Microplate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Prolactin ELISA Kit (ab272780) is intended for the quantitative determination of prolactin antigen in rat plasma.
Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti‑rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with anti-rabbit HRP secondary reagent. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin. Color development is proportional to the concentration of prolactin in the samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.
- Biological function summary
PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.
- Pathways
Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.
- Associated diseases and disorders
PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.
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ELISA - Rat Prolactin ELISA Kit (ab272780)
A typical standard curve. Example only.
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Product protocols
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