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AB113351

Rat Prolactin R ELISA Kit (PRLR)

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(2 Publications)

Rat Prolactin R ELISA Kit (PRLR) is a Sandwich (quantitative) ELISA for the measurement of Rat Prolactin R (PRLR) in Rat in Cell Culture Media samples.

View Alternative Names

Prolactin receptor, PRL-R, Lactogen receptor, Prlr

1 Images
Sandwich ELISA - Rat Prolactin R ELISA Kit (PRLR) (AB113351)
  • sELISA

Unknown

Sandwich ELISA - Rat Prolactin R ELISA Kit (PRLR) (AB113351)

Representative standard curve using ab113351

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 0.4 ng/mL

Range

0.512 - 50 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Prolactin R Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Prolactin R in cell culture supernatants.

This assay employs an antibody specific for rat Prolactin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Prolactin R antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Rat Prolactin R in normal serum with ab113351, only in serum spiked with Rat Prolactin R.

Note: very low signal were obtained in normal plasma.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "117 - 141 %", "average": "= 125.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113351-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113351-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113351-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB113351-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113351-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB113351-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113351-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat Prolactin R Standard (lyophilized)", "number":"AB113351-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Prolactin R Microplate (12 x 8 wells)", "number":"AB113351-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat Prolactin R", "number":"AB113351-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.
Biological function summary

The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.

Pathways

PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.

Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.
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Product protocols

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Target data

This is a receptor for the anterior pituitary hormone prolactin.
See full target information Prlr
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Andrologia 49: PubMed27546232

2016

6-Gingerol-rich fraction from Zingiber officinale ameliorates carbendazim-induced endocrine disruption and toxicity in testes and epididymis of rats.

Applications

Unspecified application

Species

Unspecified reactive species

M Salihu,B O Ajayi,I A Adedara,D de Souza,J B T Rocha,E O Farombi

Archives of environmental contamination and toxico 68:74-82 PubMed25179371

2014

Municipal landfill leachate-induced testicular oxidative damage is associated with biometal accumulation and endocrine disruption in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Isaac A Adedara,Ifeoluwa O Awogbindin,Adebayo A Adesina,Oluwatosin O Oyebiyi,Tajudeen A Lawal,Ebenezer O Farombi
View all publications
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