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AB238259

Rat Prolactin Receptor / PRL-R ELISA Kit

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Rat Prolactin Receptor / PRL-R ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Prolactin Receptor / PRL-R with a sensitivity of 3.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Prolactin receptor, PRL-R, Lactogen receptor, Prlr

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Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

Interpolated concentrations of spike Prolactin R in cell culture media samples. The concentrations of Prolactin R were measured in duplicates, interpolated from the Prolactin R standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

Interpolated concentrations of native Prolactin R in rat kidney and liver tissue extract samples, samples based on a 500 μg/mL and 1500 µg/mL extract load (respectively).

The concentrations of Prolactin R were measured in duplicate and interpolated from the Prolactin R standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin R concentration was determined to be 1.2 pg/μg rat kidney tissue extract and 0.15 pg/μg rat liver tissue extract.

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

Example of rat Prolactin R standard curve in Sample Diluent NS. The Prolactin R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

Interpolated concentrations of native Prolactin R in rat serum and plasma samples. The concentrations of Prolactin R were measured in duplicates, interpolated from the Prolactin R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin R concentration was determined to be 122 pg/mL in serum, 79 pg/mL in plasma (citrate), 82 pg/mL in plasma (EDTA) and 111 pg/mL in plasma (heparin).

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

Example of rat Prolactin R standard curve in 1X Cell Extraction Buffer PTR. The Prolactin R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 3.3 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat Prolactin Receptor / PRL-R ELISA Kit (ab238259) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Prolactin Receptor / PRL-R protein in cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Prolactin Receptor / PRL-R with 3.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]
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Recovery

[ { "sample": "Serum", "range": "76 - 82 %", "average": "= 78" }, { "sample": "EDTA Plasma", "range": "91 - 101 %", "average": "= 95" }, { "sample": "Tissue Extracts", "range": "94 - 103 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "81 - 82 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "83 - 95 %", "average": "= 87" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.
Biological function summary

The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.

Pathways

PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.

Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.
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Product protocols

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Target data

This is a receptor for the anterior pituitary hormone prolactin.
See full target information Prlr
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websiteProtocolBooklet
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Product promise

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