Rat RANKL (TNFSF11) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat RANKL (TNFSF11) in Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum samples.
Colorimetric
Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum
Sandwich (quantitative)
Rat
23.438 - 1500 pg/mL
1h 30m
= 3 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE
Rat RANKL (TNFSF11) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat RANKL (TNFSF11) in Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum samples.
Colorimetric
Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum
Sandwich (quantitative)
Rat
23.438 - 1500 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 3 pg/mL
102%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.9 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 102 | Range 95 - 109 % |
Sample type Heparin Plasma | Average % = 102 | Range 99 - 105 % |
Sample type Citrate plasma | Average % = 109 | Range 105 - 114 % |
Sample type EDTA Plasma | Average % = 93 | Range 86 - 102 % |
Sample type Cell culture supernatant | Average % = 107 | Range 100 - 119 % |
Blue Ice
+4°C
+4°C
+4°C
Rat RANKL (TNFSF11) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TNFSF11) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Rat RANKL (TNFSF11) with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Example of Rat RANKL standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentration of native RANKL was measured in duplicate at different sample concentrations. Undiluted samples are 100% rat splenocyte treated with concanavalin A (5µg/ml, 48hr) and 100% untreated rat splenocyte supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.
Recombinant RANKL was spiked into the following Rat samples and diluted in a 2-fold dilution series in Sample Diluent 25BS. Undiluted samples are 50% Rat Plasma (citrate), Rat Plasma (EDTA), and Rat Plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.
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