AB309285

Rat RANKL (TNFSF11) ELISA Kit

Recombinant
SimpleStep
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Rat RANKL (TNFSF11) ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat RANKL (TNFSF11) with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD254, Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE

3 Images

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

sELISA

Supplier Data

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Interpolated concentration of native RANKL was measured in duplicate at different sample concentrations. Undiluted samples are 100% rat splenocyte treated with concanavalin A (5µg/ml, 48hr) and 100% untreated rat splenocyte supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

sELISA

Supplier Data

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Recombinant RANKL was spiked into the following Rat samples and diluted in a 2-fold dilution series in Sample Diluent 25BS. Undiluted samples are 50% Rat Plasma (citrate), Rat Plasma (EDTA), and Rat Plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

sELISA

Supplier Data

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Example of Rat RANKL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat RANKL (TNFSF11) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TNFSF11) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Rat RANKL (TNFSF11) with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Serum", "range": "95 - 109 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "105 - 114 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "86 - 102 %", "average": "= 93" }, { "sample": "Cell culture supernatant", "range": "100 - 119 %", "average": "= 107" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.

See full target information Tnfsf11

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat RANKL (TNFSF11) ELISA Kit

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Assay Kits

Secondary Antibodies

Product promise