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Rat RANKL (TNFSF11) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat RANKL (TNFSF11) in Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum samples.

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Images

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285), expandable thumbnail
  • Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285), expandable thumbnail
  • Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Sensitivity

= 3 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.

Alternative names

What's included?

1 x 96 Tests
Components
10X Rat RANKL (TNFSF11) Capture Antibody
1 x 600 µL
10X Rat RANKL (TNFSF11) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Plate Seals
1 x 1 Unit
Rat RANKL (TNFSF11) Lyophilized Recombinant Protein
2 x 1 Vial
Sample Diluent 25BS
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 1 Unit
TMB Development Solution
1 x 12 mL

Recommended products

Rat RANKL (TNFSF11) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat RANKL (TNFSF11) in Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 3 pg/mL

General recovery

102%

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

3.9

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

3.9

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 102

Range

95 - 109 %

Sample type

Heparin Plasma

Average %

= 102

Range

99 - 105 %

Sample type

Citrate plasma

Average %

= 109

Range

105 - 114 %

Sample type

EDTA Plasma

Average %

= 93

Range

86 - 102 %

Sample type

Cell culture supernatant

Average %

= 107

Range

100 - 119 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Rat RANKL (TNFSF11) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TNFSF11) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Rat RANKL (TNFSF11) with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (ab309285), expandable thumbnail

    Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (ab309285)

    Example of Rat RANKL standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

  • Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (ab309285), expandable thumbnail

    Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (ab309285)

    Interpolated concentration of native RANKL was measured in duplicate at different sample concentrations. Undiluted samples are 100% rat splenocyte treated with concanavalin A (5µg/ml, 48hr) and 100% untreated rat splenocyte supernatant.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS. 

  • Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (ab309285), expandable thumbnail

    Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (ab309285)

    Recombinant RANKL was spiked into the following Rat samples and diluted in a 2-fold dilution series in Sample Diluent 25BS. Undiluted samples are 50% Rat Plasma (citrate), Rat Plasma (EDTA), and Rat Plasma (heparin).  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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