AB289699

Rat Resistin ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rat Resistin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Resistin with a sensitivity of 12.604 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 Images

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

sELISA

Supplier Data

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

Interpolated concentrations of rat Resistin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 2%, plasma (citrate) 2%, plasma (EDTA) 2%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

Example of rat Resistin standard curve in Sample Diluent NS.

Example of rat Resistin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

Interpolated concentrations of spiked rat Resistin in cell culture media (10F RPMI).

Recombinant Resistin was spiked into the following rat samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% cell culture media (10F RPMI). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.604 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Rat Resistin ELISA Kit (ab289699) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Resistin in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Resistin with 12.604 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "98 - 114 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "92 - 119 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "81 - 117 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "106 - 117 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "89 - 117 %", "average": "= 103" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289699-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289699-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289699-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289699-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289699-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289699-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289699-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB289699-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Resistin Capture Antibody", "number":"AB289699-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Resistin Lyophilized Recombinant Protein", "number":"AB289699-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Resistin Detector Antibody", "number":"AB289699-CMP08", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.

Biological function summary

Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.

Pathways

Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.

Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.

style

left-column

Product protocols

style

left-column

Target data

See full target information Retn

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:12914 PubMed40234489

2025

Memantine abrogates testicular dysfunction induced by risperidone in rats with a potential role of ERK1/2-Nrf2-caspase-3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Reham H Mohyeldin,Ehab E Sharata,Michael Atef Fawzy,Mina Ezzat Attya,Nermeen N Welson,Remon Roshdy Rofaeil

Journal of hypertension 43:752-767 PubMed39927881

2025

Thermoneutral-housed rats demonstrate impaired perivascular adipose tissue and vascular crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa M Henckel,Ji Hye Chun,Leslie A Knaub,Gregory B Pott,Georgia E James,Kendall S Hunter,Robin Shandas,Lori A Walker,Jane E-B Reusch,Amy C Keller

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Resistin ELISA Kit

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

Sandwich ELISA - Rat Resistin ELISA Kit (AB289699)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise