Rat Retinol Binding Protein 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Retinol Binding Protein 4 with a sensitivity of 3.07 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Toxicology reports 8:463-4802021Effect of embedded metal fragments on urinary metal levels and kidney biomarkers in the Sprague-Dawley rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica F Hoffman,Vernieda B Vergara,Anya X Fan,John F KalinichPubMed 33717999
- Toxicological sciences : an official journal of the Society of Toxicology 175:75-862020Impaired Tubular Reabsorption Is the Main Mechanism Explaining Increases in Urinary NGAL Excretion Following Acute Kidney Injury in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra M Sancho-Martínez,Víctor Blanco-Gozalo,Yaremi Quiros,Laura Prieto-García,María J Montero-Gómez,Neil G Docherty,Carlos Martínez-Salgado,Ana I Morales,José M López-Novoa,Francisco J López-HernándezPubMed 32110797
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 13.3 - 850 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.07 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues (PubMed:4708098). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (By similarity).
Alternative names
Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP, Rbp4
What's included?
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Rat Retinol Binding Protein 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Retinol Binding Protein 4 with a sensitivity of 3.07 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 13.3 - 850 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 3.07 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 3.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 2.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 116 | Range 109 - 126 % |
Sample type Serum | Average % = 113 | Range 107 - 116 % |
Sample type Urine | Average % = 104 | Range 103 - 105 % |
Sample type Citrate plasma | Average % = 100 | Range 91 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Retinol Binding Protein 4 ELISA Kit (ab203362) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Retinol Binding Protein 4 protein in cell culture supernatant, serum, urine, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Retinol Binding Protein 4 with 3.07 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Retinol-Binding Protein 4 (RBP4) is a small, highly conserved member of the lipocalin superfamily involved in the transportation of retinol (Vitamin A) in blood. Synthesized in hepatocytes and adipocytes, RBP4 is also an adipokine involved in insulin resistance in the AG4KO mouse model. Elevated serum levels of RBP4 are seen in obesity and Type 2 diabetes.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
- Biological function summary
RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.
- Pathways
RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.
- Associated diseases and disorders
RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
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2 product images
Sandwich ELISA - Rat Retinol Binding Protein 4 ELISA Kit (ab203362)
Example of RBP4 standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Retinol Binding Protein 4 ELISA Kit (ab203362)
Interpolated concentrations of RBP4 in rat plasma (citrate) and serum.
The concentrations of RBP4 were measured in duplicate and interpolated from the RBP4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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