Rat Sclerostin ELISA Kit (SOST)
- Recombinant
- SimpleStep
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(1 Publication)
Rat Sclerostin ELISA Kit (SOST) is a single-wash 90-min Simplestep used to quantify Rat Sclerostin (SOST) with a sensitivity of 13 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Sclerostin, Sost
- sELISA
Supplier Data
Sandwich ELISA - Rat Sclerostin ELISA Kit (SOST) (AB264622)
Rat Sclerostin standard curve comparison.
Standard Curve comparison between Rat Sclerostin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows compareable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Rat Sclerostin ELISA Kit (SOST) (AB264622)
Example of rat Sclerostin standard curve in 1X Cell Extraction Buffer PTR.
The Sclerostin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Rat Sclerostin ELISA Kit (SOST) (AB264622)
Interpolated concentrations of recombinant Sclerostin spiked into rat serum and plasma samples.
The concentrations of Sclerostin were measured in duplicates, interpolated from the Sclerostin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (heparin) 25%, plasma (citrate) 25%, and plasma (edta) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Reactivity data
Product details
Rat Sclerostin ELISA Kit (SOST) (ab264622) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Sclerostin (SOST) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Sclerostin (SOST) with 13 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Sclerostin (SOST) is a key player in bone formation. With its signaling partners, SOST inhibits Wnt signaling pathway and leads to a reduction in bone formation. Mutation of its controlling gene, SOST, can lead to a rare bone overgrowth disorder called Van Buchem disease. Anti-sclerostin antibodies are currently under investigation as a treatment for osteoporosis. The antibodies for this kit were raised against the full-length mature protein, which shares 95% and 89% identity with the mouse and human proteins, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
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Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Sclerostin inhibits osteoblastic activity and formation by interfering with bone morphogenetic proteins. It doesn’t form part of a large protein complex but interacts directly with cell surface receptors. Its expression decreases in response to mechanical loading on bones which is an important part of regulating bone density and remodeling. By controlling osteoblast function sclerostin contributes to skeletal homeostasis an essential process for maintaining bone strength and integrity.
Pathways
Several mechanisms regulate sclerostin activity. In the canonical Wnt/β-catenin signaling pathway it serves as an important checkpoint that ensures controlled bone formation. Sclerostin acts by preventing the activation of LRP5/6 receptors pivotal components in this pathway. Additionally it shares a regulatory network with dickkopf-1 (DKK1) another Wnt inhibitor both of which have an influence on bone metabolism and development.
Product protocols
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Publications (1)
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Toxicological sciences : an official journal of the Society of Toxicology 185:232-245 PubMed34755883
2021
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