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AB171334

Rat sE-Selectin ELISA Kit (sCD62E)

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(1 Publication)

Rat sE-Selectin ELISA Kit (sCD62E) is a Sandwich (quantitative) ELISA for the measurement of Rat sE-Selectin (sCD62E) in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

CD62E, Elam-1, Sele, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

1 Images
Sandwich ELISA - Rat sE-Selectin ELISA Kit (sCD62E) (AB171334)
  • sELISA

Supplier Data

Sandwich ELISA - Rat sE-Selectin ELISA Kit (sCD62E) (AB171334)

Representative Standard Curve using ab171334.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's rat soluble sE-Selectin (sCD62E) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat soluble sE-Selectin in cell culture supernatants, serum and plasma (heparin, citrate).

An sE-Selectin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for sE-Selectin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat sE-Selectin amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "345 pg/mL", "standardDeviation": "17.68", "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "149.13 pg/mL", "standardDeviation": "149.13", "coefficientOfVariability": "= 9.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1737 pg/mL", "standardDeviation": "132.01", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "309.85 pg/mL", "standardDeviation": "309.85", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4428 pg/mL", "standardDeviation": "292.24", "coefficientOfVariability": "= 6.6" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "14.8 pg/mL", "standardDeviation": "14.8", "coefficientOfVariability": "= 4.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171334-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171334-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-rat sE-Selectin antibody", "number":"AB171334-CMP09", "productcode":"" }, { "size":"1 x 96 Test", "name":"Anti-rat sE-Selectin Antibody Microplate (12 x 8 wells)", "number":"AB171334-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171334-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171334-CMP10", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171334-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171334-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171334-CMP07", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant rat sE-Selectin standard", "number":"AB171334-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171334-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171334-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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Product protocols

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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
See full target information Sele
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0132497 PubMed26196679

2015

Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-γ, NF-κB and Nrf2/HO-1 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Magdy Mahmoud-Awny,Ahmed S Attia,Mohamed F Abd-Ellah,Hanan Salah El-Abhar
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websiteProtocolBooklet
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