Rat Serpin A12 ELISA Kit

Product details

Rat Serpin A12 ELISA kit (ab272197) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Serpin A12 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Serpin A12 with 3.79 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Serpins are a member of the serine protease inhibitor superfamily proteins that share similar structures. Serpin A12, also known as vaspin, was first identified in white adipose tissue of the OLETF (Otsuka Long-Evans Tokushima Fatty) rat Type 2 diabetes model. Moreover, as an adipokine, Serpin A12 modulates insulin action by specifically inhibiting its target protease KLK7. Studies have shown Serpin A12 to have multiple beneficial effects on metabolic, inflammatory, and atherogenic processes related to obesity.