Rat Serpin A12 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Serpin A12 with a sensitivity of 3.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 23.44 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.79 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
Alternative names
Serpin A12, Visceral adipose tissue-derived serine protease inhibitor, Visceral adipose-specific serpin, Vaspin, Serpina12
What's included?
Recommended products
Rat Serpin A12 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Serpin A12 with a sensitivity of 3.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 23.44 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.79 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 86 | Range 83 - 90 % |
Sample type EDTA Plasma | Average % = 92 | Range 82 - 96 % |
Sample type Citrate plasma | Average % = 89 | Range 85 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Serpin A12 ELISA kit (ab272197) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Serpin A12 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Serpin A12 with 3.79 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Serpins are a member of the serine protease inhibitor superfamily proteins that share similar structures. Serpin A12, also known as vaspin, was first identified in white adipose tissue of the OLETF (Otsuka Long-Evans Tokushima Fatty) rat Type 2 diabetes model. Moreover, as an adipokine, Serpin A12 modulates insulin action by specifically inhibiting its target protease KLK7. Studies have shown Serpin A12 to have multiple beneficial effects on metabolic, inflammatory, and atherogenic processes related to obesity.
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2 product images
Sandwich ELISA - Rat Serpin A12 ELISA Kit (ab272197)
Example of rat Serpin A12 standard curve in Sample Diluent NS.
The Serpin A12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Serpin A12 ELISA Kit (ab272197)
Interpolated concentrations of native Serpin A12 in rat serum samples.
The concentrations of Serpin A12 were measured in duplicates, interpolated from the Serpin A12 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 489.1 pg/mL in serum.
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