JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213923

Rat TIMP-2 ELISA Kit

5

(1 Review)

|

(1 Publication)

Rat TIMP-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat TIMP-2 in Rat in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Timp-2, Timp2, Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP-2

1 Images
Sandwich ELISA - Rat TIMP-2 ELISA Kit (AB213923)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TIMP-2 ELISA Kit (AB213923)

Rat TIMP-2 ELISA Kit (ab213923) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Rat TIMP-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213923) is designed for the quantitative measurement of Rat TIMP-2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TIMP-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat TIMP-2 amount of sample captured in plate.

TIMP-2 gene is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "227 pg/mL", "standardDeviation": "10.21", "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1463 pg/mL", "standardDeviation": "95.09", "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4769 pg/mL", "standardDeviation": "443.51", "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "240 pg/mL", "standardDeviation": "10.56", "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1342 pg/mL", "standardDeviation": "68.44", "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4533 pg/mL", "standardDeviation": "339.97", "coefficientOfVariability": "7.5" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213923-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213923-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- rat TIMP-2 antibody", "number":"AB213923-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-rat TIMP-2 coated Microplate (12 x 8 wells)", "number":"AB213923-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213923-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213923-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213923-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213923-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213923-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant rat TIMP-2 standard", "number":"AB213923-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213923-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213923-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
style
left-column

Product protocols

style
left-column

Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
See full target information Timp2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 14:1252470 PubMed38173933

2024

Cysteine and glycine-rich protein 3 (Crp3) as a critical regulator of elastolysis, inflammation, and smooth muscle cell apoptosis in abdominal aortic aneurysm development.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Barbosa Marcondes de Mattos,Joao Carlos Ribeiro-Silva,Miriam Helena Fonseca-Alaniz,Iuri Cordeiro Valadão,Erasmo Simão da Silva,Jose Eduardo Krieger,Ayumi Aurea Miyakawa
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB1828

Anti-TIMP2 antibody [3A4]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (AB1828)

  • 4
  • 7
Primary Antibodies

AB283575

Anti-MMP9 antibody [RM1020]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [RM1020] (AB283575)

  • 0
  • 0
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com