JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100782

Rat TIMP1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Rat TIMP1 ELISA Kit is a Sandwich ELISA for the measurement of Rat TIMP1 in Rat in Biofluids, Cell Culture Media samples.
2 Images
Sandwich ELISA - Rat TIMP1 ELISA Kit (AB100782)
  • sELISA

Unknown

Sandwich ELISA - Rat TIMP1 ELISA Kit (AB100782)

Representative standard curve using ab100782

Sandwich ELISA - Rat TIMP1 ELISA Kit (AB100782)
  • sELISA

Unknown

Sandwich ELISA - Rat TIMP1 ELISA Kit (AB100782)

Typical data B.

Representative standard curve using ab100782

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 4 pg/mL

Range

4.1 - 3000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

  • 5
  • 1
Assay Kits

AB219046

Human Fibronectin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Fibronectin ELISA Kit (AB219046)

  • 0
  • 0
Assay Kits

AB246539

Human MMP9 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MMP9 ELISA Kit (AB246539)

  • 5
  • 1
Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's TIMP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat TIMP1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for rat TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat TIMP- 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision & recovery information

Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 93.26" }, { "sample": "Serum", "range": "85 - 104 %", "average": "= 92.79" }, { "sample": "Plasma", "range": "84 - 103 %", "average": "= 94.65" } ]

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100782-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100782-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100782-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100782-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TIMP1 Microplate (12 x 8 wells)", "number":"AB100782-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100782-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100782-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat TIMP1 Standard (lyophilized)", "number":"AB100782-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat TIMP1", "number":"AB100782-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100782-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
style
left-column

Product protocols

style
left-column

Target data

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.
See full target information Timp1

Alternative Names

Timp-1, Timp1, Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com