Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Plasma, Cell culture supernatant, Serum samples.
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- Brain, behavior, and immunity 108:162-1752022Maternal behaviours and adult offspring behavioural deficits are predicted by maternal TNFα concentration in a rat model of neurodevelopmental disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHarry G Potter,Hager M Kowash,Rebecca M Woods,Grace Revill,Amy Grime,Brendan Deeney,Matthew A Burgess,Toby Aarons,Jocelyn D Glazier,Joanna C Neill,Reinmar HagerPubMed 36503051
- International journal of environmental research and public health 19:2022Vitamin D and Swimming Exercise Prevent Obesity in Rats under a High-Fat Diet via Targeting FATP4 and TLR4 in the Liver and Adipose Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEman Kolieb,Shymaa Ahmed Maher,Mohammed Nader Shalaby,Amnah Mohammed Alsuhaibani,Afaf Alharthi,Wael A Hassan,Karima El-SayedPubMed 36360622
- Clinical science (London, England : 1979) 136:1117-11372022Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHager M Kowash,Harry G Potter,Rebecca M Woods,Nick Ashton,Reinmar Hager,Joanna C Neill,Jocelyn D GlazierPubMed 35852150
- Toxicon : official journal of the International So 166:1-82019Toxicity of Vipera palaestinae venom and antagonistic effects of methanolic leaf extract of Eryngium creticum lam.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShreen Deeb Nusair et. al.PubMed 31095960
- Acta cirurgica brasileira 32:1045-10552018Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Zhang,Yu-Jie LiuPubMed 29319733
- Evidence-based complementary and alternative medic 2018:36741452018Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaoheng Liu et. al.PubMed 29541141
- Mycotoxin research 32:53-602016Changes in serum cytokine levels, hepatic and intestinal morphology in aflatoxin B1-induced injury: modulatory roles of melatonin and flavonoid-rich fractions from Chromolena odorata.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFadeyemi Joseph Akinrinmade et. al.PubMed 26798045
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 82.3 - 20000 pg/mL
- Sensitivity
- < 25 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
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Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 82.3 - 20000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 25 pg/mL
- General recovery
- 96%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 101.54 | Range 89 - 108 % |
Sample type Serum | Average % = 95.43 | Range 84 - 104 % |
Sample type Plasma | Average % = 93.79 | Range 82 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's TNF alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.
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3 product images
ELISA - Rat TNF alpha ELISA Kit (ab100784)
Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (duplicates +/- SD).
Sandwich ELISA - Rat TNF alpha ELISA Kit (ab100784)
Representative Standard Curve using ab100784.
Sandwich ELISA - Rat TNF alpha ELISA Kit (ab100784)
Representative Standard Curve using ab100784.
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