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AB100784

Rat TNF alpha ELISA Kit

5

(1 Review)

|

(7 Publications)

Rat TNF alpha ELISA Kit is a Sandwich ELISA for the measurement of Rat TNF alpha in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

3 Images
ELISA - Rat TNF alpha ELISA Kit (AB100784)
  • ELISA

Lab

ELISA - Rat TNF alpha ELISA Kit (AB100784)

Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (duplicates +/- SD).

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100784)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100784)

Representative Standard Curve using ab100784.

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100784)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100784)

Representative Standard Curve using ab100784.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 25 pg/mL

Range

82.3 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNF alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 108 %", "average": "= 101.54" }, { "sample": "Serum", "range": "84 - 104 %", "average": "= 95.43" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 93.79" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100784-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100784-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100784-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat TNF alpha", "number":"AB100784-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100784-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat TNF alpha Standard (lyophilized)", "number":"AB100784-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100784-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 wells)", "number":"AB100784-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100784-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100784-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Brain, behavior, and immunity 108:162-175 PubMed36503051

2022

Maternal behaviours and adult offspring behavioural deficits are predicted by maternal TNFα concentration in a rat model of neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Harry G Potter,Hager M Kowash,Rebecca M Woods,Grace Revill,Amy Grime,Brendan Deeney,Matthew A Burgess,Toby Aarons,Jocelyn D Glazier,Joanna C Neill,Reinmar Hager

International journal of environmental research and public health 19: PubMed36360622

2022

Vitamin D and Swimming Exercise Prevent Obesity in Rats under a High-Fat Diet via Targeting FATP4 and TLR4 in the Liver and Adipose Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Kolieb,Shymaa Ahmed Maher,Mohammed Nader Shalaby,Amnah Mohammed Alsuhaibani,Afaf Alharthi,Wael A Hassan,Karima El-Sayed

Clinical science (London, England : 1979) 136:1117-1137 PubMed35852150

2022

Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth.

Applications

Unspecified application

Species

Unspecified reactive species

Hager M Kowash,Harry G Potter,Rebecca M Woods,Nick Ashton,Reinmar Hager,Joanna C Neill,Jocelyn D Glazier

Toxicon : official journal of the International So 166:1-8 PubMed31095960

2019

Toxicity of Vipera palaestinae venom and antagonistic effects of methanolic leaf extract of Eryngium creticum lam.

Applications

Unspecified application

Species

Unspecified reactive species

Shreen Deeb Nusair,Mohammad Ibrahim Ahmad

Acta cirurgica brasileira 32:1045-1055 PubMed29319733

2018

Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Yu-Jie Liu

Evidence-based complementary and alternative medic 2018:3674145 PubMed29541141

2018

Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoheng Liu,Wei Wang,Fang Cao,Shuo Liu,Xinxin Zou,Guodong Li,Haojie Yang,Yang Jiao

Mycotoxin research 32:53-60 PubMed26798045

2016

Changes in serum cytokine levels, hepatic and intestinal morphology in aflatoxin B1-induced injury: modulatory roles of melatonin and flavonoid-rich fractions from Chromolena odorata.

Applications

Unspecified application

Species

Unspecified reactive species

Fadeyemi Joseph Akinrinmade,Akinleye Stephen Akinrinde,Adetayo Amid
View all publications
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Product promise

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