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Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Cell culture extracts, Tissue Extracts, Cell culture supernatant samples.
Alternative names=Thymidine kinase, TK
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
82.3 - 20000 pg/mL
- Sensitivity
< 25 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Cell culture extracts, Tissue Extracts, Cell culture supernatant samples.
Alternative names=Thymidine kinase, TK
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
82.3 - 20000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 25 pg/mL
- General recovery
92%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 93.17 | Range 81 - 105 % |
Sample type Tissue Extracts | Average % = 92.48 | Range 80 - 104 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's TNF alpha ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in cell lysates and tissue lysates.
This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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