JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100785

Rat TNF alpha ELISA Kit

4

(2 Reviews)

|

(107 Publications)

Rat TNF alpha ELISA Kit is a Sandwich ELISA for the measurement of Rat TNF alpha in Rat in Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

Thymidine kinase, TK

3 Images
Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100785)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100785)

Representative standard curve using ab100785

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100785)
  • sELISA

Unknown

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100785)

Rat TNF alpha detected in cell lysates from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma). Results shown for TNF alpha levels in 10e6 cells after background signal was subtracted (duplicates +/- SD).

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100785)
  • sELISA

Unknown

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB100785)

Rat TNF alpha detected in cell supernatants from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma); background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 25 pg/mL

Range

82.3 - 20000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2
Assay Kits

AB255730

Rat IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Rat IL-1 beta ELISA Kit (AB255730)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB214566

Rat IL-10 ELISA Kit

Recombinant|SimpleStep

ELISA - Rat IL-10 ELISA Kit (AB214566)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's TNF alpha ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in cell lysates and tissue lysates.

This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "81 - 105 %", "average": "= 93.17" }, { "sample": "Tissue Extracts", "range": "80 - 104 %", "average": "= 92.48" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100785-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100785-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100785-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100785-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100785-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100785-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat TNF alpha", "number":"AB100785-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100785-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 wells)", "number":"AB100785-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat TNF alpha Standard (lyophilized)", "number":"AB100785-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100785-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

See full target information TK
style
left-column

Publications (107)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40508105

2025

Niacin Modulates SIRT1-Driven Signaling to Counteract Radiation-Induced Neurocognitive and Behavioral Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Erdinç Tunç,Hatice Aygün,Mümin Alper Erdoğan,Yiğit Uyanıkgil,Oytun Erbaş

Frontiers in cardiovascular medicine 12:1515160 PubMed40236258

2025

Sevoflurane pretreatment alleviates hypoxia-reoxygenation-induced myocardial cell injury by upregulating miR-21-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Saiwen Qiu,Hui Chen,Qifang Jiang

Diabetes, metabolic syndrome and obesity : targets and therapy 17:4867-4882 PubMed39742288

2025

Morin Ameliorates Myocardial Injury in Diabetic Rats via Modulation of Autophagy, Apoptosis, Inflammation, and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhu,Jizhong He

Journal of biochemical and molecular toxicology 38:e70082 PubMed39651608

2024

Vitamin D3 Exerts a Neuroprotective Effect in Metabolic Syndrome Rats: Role of BDNF/TRKB/Akt/GS3Kβ Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Noha Aladdin,Salah A Ghareib

Reproduction & fertility 5: PubMed39374132

2024

MICROBIOME: The trials and errors of developing an experimental model to study the impact of maternal gut microbiome disruption on perinatal asphyxia.

Applications

Unspecified application

Species

Unspecified reactive species

Mara Ioana Ionescu,Ana Maria Catrina,Ioana Alexandra Dogaru,Didina Catalina Barbalata,Cristian Ciotei,Cerasela Haidoiu,Vladimir Suhaianu,Gratiela Gradisteanu Pircalabioru,Siobhain M O'Mahony,Ana-Maria Zagrean

Antioxidants (Basel, Switzerland) 13: PubMed39334757

2024

L. (Safflower) Flower Extract Attenuates Hepatic Injury and Steatosis in a Rat Model of Type 2 Diabetes Mellitus via Nrf2-Dependent Hypoglycemic, Antioxidant, and Hypolipidemic Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Nuha Saad Alshareef,Sahar Abdulaziz AlSedairy,Laila Naif Al-Harbi,Ghedeir M Alshammari,Mohammed Abdo Yahya

Nutrients 16: PubMed39339674

2024

Comparative Efficacy of Low-Carbohydrate and Ketogenic Diets on Diabetic Retinopathy and Oxidative Stress in High-Fat Diet-Induced Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Monya T Jawharji,Ghedeir M Alshammari,Manal Abdulaziz Binobead,Nouf Mohammed Albanyan,Laila Naif Al-Harbi,Mohammed Abdo Yahya

PloS one 19:e0301883 PubMed39292699

2024

PDE5 inhibitor potentially improves polyuria and bladder storage and voiding dysfunctions in type 2 diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Kabuto,So Inamura,Hisato Kobayashi,Xinmin Zha,Keiko Nagase,Minekatsu Taga,Masaya Seki,Nobuki Tanaka,Yoshinaga Okumura,Osamu Yokoyama,Naoki Terada

The Journal of international medical research 52:3000605241253733 PubMed38811356

2024

Effect of high-dose intravenous iron injection on hepatic function in a rat model of cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hye Kwon,RyungA Kang,Sangmin Maria Lee,Tae Soo Hahm,Hyun Sung Cho,Gayoung Jin,Justin Sangwook Ko

Frontiers in pharmacology 15:1343941 PubMed38549671

2024

CHF6297: a novel potent and selective p38 MAPK inhibitor with robust anti-inflammatory activity and suitable for inhaled pulmonary administration as dry powder.

Applications

Unspecified application

Species

Unspecified reactive species

Cataldo Martucci,Andrew Dennis Allen,Nadia Moretto,Valentina Bagnacani,Alessandro Fioni,Riccardo Patacchini,Maurizio Civelli,Gino Villetti,Fabrizio Facchinetti
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com