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AB236712

Rat TNF alpha ELISA Kit

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(75 Publications)

Rat TNF alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat TNF alpha with a sensitivity of 1.04 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 60 citations

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

4 Images
ELISA - Rat TNF alpha ELISA Kit (AB236712)
  • ELISA

Supplier Data

ELISA - Rat TNF alpha ELISA Kit (AB236712)
Sandwich ELISA - Rat TNF alpha ELISA Kit (AB236712)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB236712)

Interpolated concentrations of native TNF alpha in rat splenocyte cell culture supernatant based on 100% load.

The concentrations of TNF alpha were measured in duplicate and interpolated from the TNF alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was below the lowest standard (<18.75) pg/mL in unstimulated splenocyte cell culture supernatant and 156.05 pg/mL in stimulated splenocyte cell culture supernatant. Stimulated samples were treated with 50 ng/mL PMA + 500 ng/mL calcium ionomycin for 72 hours. Unstimulated cells as a control were cultured in parallel for 72 hours.

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB236712)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB236712)

Interpolated concentrations of native TNF alpha in rat cell culture supernatant samples.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are 50 ng/mL PMA + 500 ng/mL calcium ionomycin treated splenocyte cell culture supernatant - 100%. Treatment length was 72 hours. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 156.05 pg/mL in treated splenocyte culture supernatant.

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB236712)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB236712)

Example of rat TNF alpha standard curve in Sample Diluent NS.

The TNF alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 1.04 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat TNF alpha ELISA Kit ab236712 is a rapid single-wash 90-min Sandwich ELISA to measure Rat TNF alpha in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.04 pg/mL.

How the assay works

Rat TNF alpha SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat TNF alpha ELISA Kit ab236712 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TNF alpha antibody [EPR21753-82] - BSA and Azide free (Capture) ab245059
- Anti-TNF alpha antibody [EPR21753-79] - BSA and Azide free (Detector) ab245059
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 115 %", "average": "= 106" }, { "sample": "Serum", "range": "79 - 83 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "81 - 82 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "76 - 78 %", "average": "= 77" }, { "sample": "Citrate plasma", "range": "80 - 83 %", "average": "= 82" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab236712-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB236712-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236712-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB236712-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Rat TNF alpha Capture Antibody", "number":"AB236712-CMP02" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236712-CMP05" }, { "size":"2 x 10 Vial", "name":"Rat TNF alpha Lyophilized Recombinant Protein", "number":"AB236712-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB236712-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB236712-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236712-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Rat TNF alpha Detector Antibody", "number":"AB236712-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab236712-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB236712-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236712-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236712-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB236712-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB236712-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat TNF alpha Lyophilized Recombinant Protein", "number":"AB236712-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat TNF alpha Capture Antibody", "number":"AB236712-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB236712-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat TNF alpha Detector Antibody", "number":"AB236712-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236712-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
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Publications (75)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1510250 PubMed40584618

2025

The anti-depressive role of the Pei Yuan Kai Yu formula in cerebral small vessel disease based on gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Lixuan Yang,Yutian Ao,Yannan Li,Baoan Dai,Junnan Li,Wenzhe Duan,Kaiqiang Dong,Zhenyun Han,Rongjuan Guo

Journal of orthopaedic surgery and research 20:517 PubMed40420098

2025

TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li

Allergologia et immunopathologia 53:88-97 PubMed40342118

2025

Dieckol ameliorates inflammatory response via inhibition of CHI3L1 expression in collagen-induced arthritis rats.

Applications

Unspecified application

Species

Unspecified reactive species

Weijiang Wang,Huijie Li

Life (Basel, Switzerland) 15: PubMed40141836

2025

Tangeretin Unravels Metabolic Dysfunction-Associated Fatty Liver Disease in Rats by Enhancing the IRS/Akt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ittipon Yuenyong,Prapassorn Potue,Putcharawipa Maneesai,Juthamas Khamseekaew,Apiwan Manimmanakorn,Metee Iampanichakul,Poungrat Pakdeechote

Iranian journal of pharmaceutical research : IJPR 23:e148139 PubMed40066121

2025

The Glucagon-Like Peptide-1 (GLP-1) Receptor Agonist Liraglutide Regulates Sirtuin-1-Mediated Neutrophil Extracellular Traps to Improve Diabetes-Induced Bone Metabolism Imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zhong,Liangzhi Huang,Tingting Lin,Yanyan Li,Bin Deng,Dezhi Kong,Zhanlin Liao,Zugui Huang

Organogenesis 21:2460261 PubMed39967390

2025

Tetramethylpyrazine Confers Protection Against Oxidative Stress and NLRP3-Dependent Pyroptosis in Rats with Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Xu,Mingzhe Zhang,Xiaofeng Zou,Mingyang Wang

Stem cell research & therapy 16:70 PubMed39940043

2025

Pericyte-derived extracellular vesicles improve vascular barrier function in sepsis via the Angpt1/PI3K/AKT pathway and pericyte recruitment: an in vivo and in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Sen Zhang,Ao Yang,Xi Luo,He-Nan Zhou,Yi-Yan Liu,Dai-Qin Bao,Jie Zhang,Jia-Tao Zang,Qing-Hui Li,Tao Li,Liang-Ming Liu

Nutrients 17: PubMed39861396

2025

Polyphenolic Hispolon Derived from Medicinal Mushrooms of the and Genera Promotes Wound Healing in Hyperglycemia-Induced Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Shan Liu,Mei-Chou Lai,Yu-Cheng Tzeng,I-Min Liu

International heart journal 65:1144-1152 PubMed39617504

2024

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal MiR-744-5p Alleviates Obstructive Sleep Apnea-Induced Myocardial Injury by Targeting NFIX.

Applications

Unspecified application

Species

Unspecified reactive species

Jiarong Wang,Lili Huang,Yimin Chen

Molecular medicine (Cambridge, Mass.) 30:203 PubMed39508252

2024

Dexmedetomidine alleviates intestinal ischemia/reperfusion injury by modulating intestinal neuron autophagy and mitochondrial homeostasis via Nupr1 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wu,Qiuhong Chen,Sisi Liang,Jinping Nie,Yingjie Wang,Chenlu Fan,Zhen Liu,Xuekang Zhang
View all publications
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