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AB46070

Rat TNF alpha ELISA Kit

4

(1 Review)

|

(111 Publications)

Rat TNF alpha ELISA Kit is a Sandwich ELISA for the measurement of Rat TNF alpha in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

MSA2, MSP2, Merozoite surface protein 2, AG513, Merozoite 45 kDa surface antigen, Merozoite surface antigen 2, MSA-2

3 Images
ELISA - Rat TNF alpha ELISA Kit (AB46070)
  • ELISA

Lab

ELISA - Rat TNF alpha ELISA Kit (AB46070)

Standard curve with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB46070)
  • sELISA

Unknown

Sandwich ELISA - Rat TNF alpha ELISA Kit (AB46070)

Representative Standard Curve using ab46070

ELISA - Rat TNF alpha ELISA Kit (AB46070)
  • ELISA

Lab

ELISA - Rat TNF alpha ELISA Kit (AB46070)

Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA ((PMA) ab120297) or PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (LPS+PMA) for the last 6 hours (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 15 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.

A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

This kit will recognize both endogenous and recombinant rat TNF alpha

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 9, "mean": "541.4 pg/mL", "standardDeviation": "33.2", "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 9, "mean": "149.4 pg/mL", "standardDeviation": "9.8", "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 10, "mean": "130.1 pg/mL", "standardDeviation": "10.3", "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 12, "mean": "543.5 pg/mL", "standardDeviation": "44.7", "coefficientOfVariability": "8.2" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46070-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46070-CMP04" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46070-CMP07" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46070-CMP01" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46070-CMP08" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46070-CMP09" }, { "size":"4 x 1 Vial", "name":"TNF alpha Standard (Lyophilized)", "number":"AB46070-CMP11" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46070-CMP05" }, { "size":"2 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 well strips)", "number":"AB46070-CMP10" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46070-CMP03" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46070-CMP06" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-TNF alpha", "number":"AB46070-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46070-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46070-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46070-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46070-CMP09", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46070-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"TNF alpha Standard (Lyophilized)", "number":"AB46070-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46070-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 well strips)", "number":"AB46070-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46070-CMP06", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46070-CMP05", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-TNF alpha", "number":"AB46070-CMP02", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46070-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

May play a role in the merozoite attachment to the erythrocyte.
See full target information MSP2
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Publications (111)

Recent publications for all applications. Explore the full list and refine your search

Current medical science 45:288-300 PubMed40035996

2025

Safranal Ameliorates Renal Damage, Inflammation, and Podocyte Injury in Membranous Nephropathy via SIRT/NF-κB Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Bao,Ya-Mei Ge,Zheng Wang,Hong-Yun Wang,Qiong Wang,Jun Yuan

Biology 13: PubMed39596850

2024

In-Depth Analysis of L. Leaf Extract: Alleviating Pulmonary Histological Disturbances, Pro-Inflammatory Responses, and Oxidative Stress from Isolated or Combined Exposure to Inhaled Toluene and Noise in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Takoua Ben Attia,Sana Bahri,Sonia Ben Younes,Afef Nahdi,Ridha Ben Ali,Linda Bel Haj Kacem,Michèle Véronique El May,Eduardo Alberto López-Maldonado,Abada Mhamdi

Journal of biochemical and molecular toxicology 38:e23773 PubMed39030868

2024

Protective effects of 3, 4-dihydroxybenzoic acid on myocardial infarction induced by isoproterenol in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sikha Vincent,Shervin Prince Stanely,Stanely Mainzen Prince Ponnian

International journal of medical sciences 21:1756-1768 PubMed39006838

2024

Mutations Contribute to Infantile Epileptic Spasm Syndrome and Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhui Hu,Deying Liu,Hua Wang

Toxicology research 13:tfae066 PubMed38660688

2024

Ameliorative impacts of sinapic acid against mercuric chloride-induced renal toxicity: role of antioxidants and inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Arshad Mehmood,Mohamed Mohamed Soliman,Daklallah A Almalki,Khalid S Alotaibi,Gehan Basiony Ahmed Youssef,Saed Althobaiti

Biomedicines 11: PubMed38137423

2023

Protective Effect of Topiramate against Diabetic Retinopathy and Computational Approach Recognizing the Role of NLRP3/IL-1β/TNF-α Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hala M F Mohammad,Mohamed Ahmed Eladl,Asmaa K K Abdelmaogood,Rabie E Elshaer,Walaa Ghanam,Abdelhakeem Elaskary,Mohamed A K Saleh,Amira H Eltrawy,Sahar K Ali,Suzan M M Moursi,Shymaa E Bilasy,Sawsan A Zaitone,Wafa Ali Alzlaiq,Hayam Atteya

Genes and environment : the official journal of the Japanese Environmental Mutagen Society 45:24 PubMed37817266

2023

LncRNA PVT1 induces apoptosis and inflammatory response of bronchial epithelial cells by regulating miR-30b-5p/BCL2L11 axis in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Taoli Fu,Hui Tian,Hui Rong,Ping Ai,Xiaoping Li

Scientific reports 13:5005 PubMed36973339

2023

Arabic gum ameliorates systemic modulation in Alloxan monohydrate-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha Mohammed Ibrahim,Hemmat Mansour Abdelhafez,Sawsan Abd El-Maksoud El-Shamy,Fatma Ahmed Eid,Alya Mashaal

Metabolites 13: PubMed36677055

2023

Quercetin, a Plant Flavonol Attenuates Diabetic Complications, Renal Tissue Damage, Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Arshad Husain Rahmani,Mohammed A Alsahli,Amjad Ali Khan,Saleh A Almatroodi

Molecules (Basel, Switzerland) 27: PubMed36558044

2022

The Synergistic Hepatoprotective Activity of Rosemary Essential Oil and Curcumin: The Role of the MEK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged E Mohamed,Nancy S Younis,Hossam S El-Beltagi,Omar M Mohafez
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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