Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Cell culture supernatant, Serum samples.
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- Biomedicines 11:2023Protective Effect of Topiramate against Diabetic Retinopathy and Computational Approach Recognizing the Role of NLRP3/IL-1β/TNF-α Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHala M F Mohammad,Mohamed Ahmed Eladl,Asmaa K K Abdelmaogood,Rabie E Elshaer,Walaa Ghanam,Abdelhakeem Elaskary,Mohamed A K Saleh,Amira H Eltrawy,Sahar K Ali,Suzan M M Moursi,Shymaa E Bilasy,Sawsan A Zaitone,Wafa Ali Alzlaiq,Hayam AtteyaPubMed 38137423
- Genes and environment : the official journal of the Japanese Environmental Mutagen Society 45:242023LncRNA PVT1 induces apoptosis and inflammatory response of bronchial epithelial cells by regulating miR-30b-5p/BCL2L11 axis in COPD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaoli Fu,Hui Tian,Hui Rong,Ping Ai,Xiaoping LiPubMed 37817266
- Scientific reports 13:50052023Arabic gum ameliorates systemic modulation in Alloxan monohydrate-induced diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRasha Mohammed Ibrahim,Hemmat Mansour Abdelhafez,Sawsan Abd El-Maksoud El-Shamy,Fatma Ahmed Eid,Alya MashaalPubMed 36973339
- Metabolites 13:2023Quercetin, a Plant Flavonol Attenuates Diabetic Complications, Renal Tissue Damage, Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArshad Husain Rahmani,Mohammed A Alsahli,Amjad Ali Khan,Saleh A AlmatroodiPubMed 36677055
- Molecules (Basel, Switzerland) 27:2022The Synergistic Hepatoprotective Activity of Rosemary Essential Oil and Curcumin: The Role of the MEK/ERK Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaged E Mohamed,Nancy S Younis,Hossam S El-Beltagi,Omar M MohafezPubMed 36558044
- International journal of molecular sciences 23:2022Treadmill Exercise Prevents Cognitive Impairments in Adolescent Intermittent Ethanol Rats by Reducing the Excessive Activation of Microglia Cell in the Hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanxia Guo,Min Yan,Li Li,Li Zhao,Yan LiPubMed 36499029
- Molecules (Basel, Switzerland) 27:2022Essential Oil Mitigates Peptic Ulcer in Rats through Nrf2/HO-1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManar K Alomair,Lama S Alabduladheem,Marwah A Almajed,Amjad A Alobaid,Essraa A R Alkhalifah,Nancy S Younis,Maged E MohamedPubMed 36432009
- Current issues in molecular biology 44:5390-54042022Cardamom Extract Alleviates the Oxidative Stress, Inflammation and Apoptosis Induced during Acetaminophen-Induced Hepatic Toxicity via Modulating Nrf2/HO-1/NQO-1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEssraa A R Alkhalifah,Amjad A Alobaid,Marwah A Almajed,Manar K Alomair,Lama S Alabduladheem,Sarah F Al-Subaie,Abdullah Akbar,Mahesh V Attimarad,Nancy S Younis,Maged E MohamedPubMed 36354677
- Life (Basel, Switzerland) 12:2022Ameliorative Impacts of Wheat Germ Oil against Ethanol-Induced Hepatic and Renal Dysfunction in Rats: Involvement of Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSalwa A Elgendy,Samar H Baloza,Lina Abdelhady Mohammed,Hend Elsayed Nasr,Noha Osama El-Shaer,Heba I Ghamry,Saed A Althobaiti,Mustafa Shukry,Mohamed Mohamed Soliman,Heba A ElnouryPubMed 36295108
- Life (Basel, Switzerland) 12:2022Ameliorative Effect of D-Carvone against Hepatic Ischemia-Reperfusion-Induced Injury in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaged E Mohamed,Nancy S YounisPubMed 36294936
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 31.25 - 1000 pg/mL
- Assay time
- 3h 45m
- Sensitivity
- < 15 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
May play a role in the merozoite attachment to the erythrocyte.
Alternative names
MSA2, MSP2, Merozoite surface protein 2, AG513, Merozoite 45 kDa surface antigen, Merozoite surface antigen 2, MSA-2
What's included?
Recommended products
Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 31.25 - 1000 pg/mL
- Assay time
- 3h 45m
- Assay Platform
- Microplate
- Sensitivity
- < 15 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample B | n 10 | mean 130.1 pg/mL | SD 10.3 | C.V. 7.9 |
Sample Sample A | n 12 | mean 543.5 pg/mL | SD 44.7 | C.V. 8.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 9 | mean 541.4 pg/mL | SD 33.2 | C.V. 6.1 |
Sample Sample B | n 9 | mean 149.4 pg/mL | SD 9.8 | C.V. 6.5 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant rat TNF alpha
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
ELISA - Rat TNF alpha ELISA Kit (ab46070)
Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA ((PMA) Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) or PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (LPS+PMA) for the last 6 hours (duplicates +/- SD).
ELISA - Rat TNF alpha ELISA Kit (ab46070)
Standard curve with background signal subtracted (duplicates; +/- SD).
Sandwich ELISA - Rat TNF alpha ELISA Kit (ab46070)
Representative Standard Curve using ab46070
Downloads
Product protocols
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