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Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Cell culture supernatant, Serum samples.
Alternative names=Merozoite surface protein 2, AG513, Merozoite 45 kDa surface antigen, Merozoite surface antigen 2, MSA-2, MSA2, MSP2
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
31.25 - 1000 pg/mL
- Assay time
3h 45m
- Sensitivity
< 15 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat TNF alpha in Rat in Cell culture supernatant, Serum samples.
Alternative names=Merozoite surface protein 2, AG513, Merozoite 45 kDa surface antigen, Merozoite surface antigen 2, MSA-2, MSA2, MSP2
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
31.25 - 1000 pg/mL
- Assay time
3h 45m
- Assay Platform
Microplate
- Sensitivity
< 15 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample B | n 10 | mean 130.1 pg/mL | SD 10.3 | C.V. 7.9 |
Sample Sample A | n 12 | mean 543.5 pg/mL | SD 44.7 | C.V. 8.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 9 | mean 541.4 pg/mL | SD 33.2 | C.V. 6.1 |
Sample Sample B | n 9 | mean 149.4 pg/mL | SD 9.8 | C.V. 6.5 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Abcam's TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant rat TNF alpha
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
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Product protocols
- Visit the general protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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