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AB48419

Rat TNF alpha ELISA Set (without plates)

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Rat TNF alpha ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Rat TNF alpha ELISA Set (without plates) in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

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ELISA - Rat TNF alpha ELISA Set (without plates) (AB48419)
  • ELISA

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ELISA - Rat TNF alpha ELISA Set (without plates) (AB48419)

Representative Standard Curve using ab48419.

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNFα Rat in vitro ELISA Set is designed for the quantitative measurement of Rat TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

Recognizes both natural and recombinant Rat TNF alpha Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml

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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab48419-5x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB48419-CMP03" }, { "size":"1 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB48419-CMP01" }, { "size":"1 x 0.5 mL", "name":"Rat TNF alpha Capture antiboy", "number":"AB48419-CMP02" }, { "size":"5 x 1 Vial", "name":"TNF alpha Standard (Lyophilized)", "number":"AB48419-CMP04" }, { "size":"1 x 0.55 mL", "name":"Detection biotinylated anti-rat TNF alpha antibody", "number":"AB48419-CMP05" } ] }, "15x96Tests": { "sellingSize": "15 x 96 Tests", "publicAssetCode":"ab48419-15x96Tests", "assetComponentDetails": [ { "size":"6 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB48419-CMP03" }, { "size":"3 x 0.5 mL", "name":"Rat TNF alpha Capture antiboy", "number":"AB48419-CMP02" }, { "size":"3 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB48419-CMP01" }, { "size":"15 x 1 Vial", "name":"TNF alpha Standard (Lyophilized)", "number":"AB48419-CMP04" }, { "size":"3 x 0.55 mL", "name":"Detection biotinylated anti-rat TNF alpha antibody", "number":"AB48419-CMP05" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab48419-10x96Tests", "assetComponentDetails": [ { "size":"2 x 0.5 mL", "name":"Rat TNF alpha Capture antiboy", "number":"AB48419-CMP02", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB48419-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB48419-CMP01", "productcode":"" }, { "size":"10 x 1 Vial", "name":"TNF alpha Standard (Lyophilized)", "number":"AB48419-CMP04", "productcode":"" }, { "size":"2 x 0.55 mL", "name":"Detection biotinylated anti-rat TNF alpha antibody", "number":"AB48419-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
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