Rat TNFR1 ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Rat TNFR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat TNFR1 with a sensitivity of 8.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD120a, Tnfr-1, Tnfr1, Tnfrsf1a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I
- sELISA
Supplier Data
Sandwich ELISA - Rat TNFR1 ELISA Kit (AB231925)
Example of rat TNFR1 standard curve.
The TNFR1 standard curve was prepared as described in Section 10.
- sELISA
Supplier Data
Sandwich ELISA - Rat TNFR1 ELISA Kit (AB231925)
Interpolated concentrations of spiked TNFR1 in rat cell culture media samples.
The concentrations of TNFR1 were measured in duplicates, interpolated from the TNFR1 standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Rat TNFR1 ELISA Kit (AB231925)
Rat TNFR1 Competitor standard curve comparison.
Standard curve comparison between Rat TNFR1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Rat TNFR1 ELISA Kit (AB231925)
Interpolated concentrations of native TNFR1 in rat serum and plasma samples.
The concentrations of TNFR1 were measured in duplicates, interpolated from the TNFR1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFR1 concentration was determined to be 8,972 pg/mL in serum, 4,723 pg/mL in plasma (citrate), 8468 pg/mL in plasma (EDTA) and 6,505 pg/mL in plasma (heparin).
Reactivity data
Product details
Rat TNFR1 ELISA Kit (ab231925) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNFR1 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat TNFR1 with 8.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
umor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Soluble TNF Receptor I is involved in regulating inflammation. It sequesters TNF to control its bioavailability and impede its pro-inflammatory effects. sTNFR1 is part of a larger signaling complex which also includes other TNF receptors and ligands within the TNF receptor superfamily. This ability to regulate TNF activity suggests its important role in maintaining immune system balance and preventing excessive inflammation.
Pathways
Soluble TNF Receptor I modulates key inflammatory pathways including the NF-kB and MAPK pathways. These pathways are critical in the cellular response to stress apoptosis and survival. By binding TNF-alpha sTNFR1 interferes with TNF-alpha's interaction with TNFR1 and TNFR2 cell receptors which are vital in signaling through these pathways. TNFR1 particularly mediates a range of cellular responses including apoptosis and survival by influencing downstream kinases and transcription factors.
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Oncoimmunology 11:2049487 PubMed35309730
2022
Applications
Unspecified application
Species
Unspecified reactive species
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