Rat TREM1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat TREM1 in Serum, Citrate plasma, EDTA Plasma, Cell culture media, Heparin Plasma samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 7.81 - 500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.37 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
TREM1_RAT, Trem1, Triggering receptor expressed on myeloid cells 1
What's included?
Rat TREM1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat TREM1 in Serum, Citrate plasma, EDTA Plasma, Cell culture media, Heparin Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 7.81 - 500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.37 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 8 | C.V. 3.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 3 | C.V. 7.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 97 - 100 % |
Sample type Citrate plasma | Average % = 101 | Range 95 - 108 % |
Sample type EDTA Plasma | Average % = 107 | Range 103 - 109 % |
Sample type Cell culture media | Average % = 103 | Range 100 - 109 % |
Sample type Heparin Plasma | Average % = 94 | Range 89 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat TREM1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM1 protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture media. Quantitate Rat TREM1 with 1.37 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Rat TREM1 ELISA Kit (ab323607)
Example of rat TREM1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat TREM1 ELISA Kit (ab323607)
Interpolated concentrations of rat TREM1 in serum, plasma (citrate), plasma (EDTA) and plasma (heparin). Interpolated concentration of native TREM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 5%, plasma (citrate) 10%, plasma (EDTA) 8%, plasma (heparin) 10%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sampled dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat TREM1 ELISA Kit (ab323607)
Example of rat TREM1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat TREM1 ELISA Kit (ab323607)
Interpolated concentrations of rat TREM1 in serum in 96-well vs. 384-well plates. Interpolated concentration of native TREM1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% rat serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat TREM1 ELISA Kit (ab323607)
Standard curve comparison between Rat TREM1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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