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AB119609

Rat TrkA ELISA Kit

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Rat TrkA ELISA Kit is a Sandwich ELISA for the measurement of Rat TrkA in Rat in Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

Trk, Trka, Ntrk1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor, p140-TrkA, Trk-A

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Sandwich ELISA - Rat TrkA ELISA Kit (AB119609)
  • sELISA

Supplier Data

Sandwich ELISA - Rat TrkA ELISA Kit (AB119609)

Representative standard curve using ab119609

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell culture supernatant, Cell Lysate

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's rat TrkA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat TrkA in cell culture supernatants, cell lysates and tissue homogenates.

A TrkA specific rat polyclonal antibody from goat has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat TrkA amount of sample captured in plate.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkA also known as NTRK1 is a neurotrophin receptor and a member of the tropomyosin receptor kinase (TRK) family. It has a molecular mass of approximately 140 kDa. TrkA is expressed mostly in neuronal tissues particularly in the sensory ganglia sympathetic neurons and certain portions of the central nervous system. Mechanically TrkA activates upon binding to nerve growth factor (NGF) leading to receptor dimerization and autophosphorylation triggering downstream signaling cascades essential for neuronal growth and survival.
Biological function summary

TrkA plays an important role in the regulation of neurotrophic signaling. TrkA existing often as part of larger signaling complexes modulates neuronal differentiation development and apoptosis. It ensures proper neural connections during development by responding to NGF which is important for neurons' health and plasticity. TrkA also aids in the response to injury by promoting neuronal repair and regeneration.

Pathways

TrkA engagement activates prominent pathways like the MAPK/ERK and PI3K/AKT signaling cascades. These pathways facilitate important cellular processes like proliferation survival and metabolism. Through these pathways TrkA interacts and cooperates with proteins like SHC GRB2 and PLCγ among others to execute cellular functions. These signaling pathways enable the coordinated response needed for neural tissue maintenance and development.

TrkA abnormalities are linked to conditions like congenital insensitivity to pain with anhidrosis (CIPA) and certain cancers. Mutations in NTRK1 can disrupt NGF signaling leading to neurological deficits observed in CIPA. In cancer aberrant TrkA signaling often due to gene fusions contributes to tumorigenesis. TrkA-related signals influence oncogenic proteins such as RAS and BRAF illustrating its role in cancer progression.
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Product protocols

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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed : 1312719, PubMed : 1850821, PubMed : 31440771). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
See full target information Ntrk1
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Product promise

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