Rat uPA ELISA kit (total uPA antigen) is a Quantitative ELISA kit for the measurement of Rat uPA (total uPA antigen) in Rat in Cell culture media, Citrate plasma, EDTA Plasma, Serum, Tissue Extracts, Urine samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Assay type
- Quantitative
- Reactive species
- Rat
- Range
- 0.01 - 10 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Alternative names
Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Plau
What's included?
Recommended products
Rat uPA ELISA kit (total uPA antigen) is a Quantitative ELISA kit for the measurement of Rat uPA (total uPA antigen) in Rat in Cell culture media, Citrate plasma, EDTA Plasma, Serum, Tissue Extracts, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Assay type
- Quantitative
- Reactive species
- Rat
- Range
- 0.01 - 10 ng/mL
- Assay Platform
- Microplate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat uPA ELISA kit (total uPA antigen) (ab272781) is intended for the quantitative determination of total urokinase plasminogen activator in rat plasma, serum, urine, cell culture media, or tissue extracts.
Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
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1 product image
ELISA - Rat uPA ELISA kit (total uPA antigen) (ab272781)
A typical standard curve. Example only.
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Product protocols
- Visit the general protocols
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