JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB274405

Rat Uromodulin ELISA Kit

5

(1 Review)

|

(0 Publication)

Rat Uromodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Uromodulin with a sensitivity of 6.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, Umod

2 Images
Sandwich ELISA - Rat Uromodulin ELISA Kit (AB274405)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Uromodulin ELISA Kit (AB274405)

Example of rat Uromodulin standard curve in 1X Cell Extraction Buffer PTR.

The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Uromodulin ELISA Kit (AB274405)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Uromodulin ELISA Kit (AB274405)

Interpolated concentrations of native Uromodulin in rat serum, plasma and urine samples.

The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma - heparin 50%, plasma - citrate 100%, plasma - EDTA 100%, and urine 1 : 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 3.33 ng/mL in serum, 2.22 ng/mL in plasma – heparin, 1.80 ng/mL in plasma – citrate, 2.09 ng/mL in plasma - EDTA and 1,598.60 ng/mL in urine.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.9 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB207170

Anti-UMOD antibody [EPR20071]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (AB207170)

  • 5
  • 3
Assay Kits

AB241897

Human Uromodulin Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human Uromodulin Antibody Pair - BSA and Azide free (AB241897)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Primary Antibodies

AB18258

Anti-PSD95 antibody - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

  • 5
  • 40
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Rat Uromodulin ELISA kit (ab274405) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Uromodulin protein in serum, plasmas and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Uromodulin with 6.9 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Uromodulin, also known as Tamms-Horsfell protein or uromucoid, plays two distinct but important roles in biology. In the kidney, it is a key protein which forms the water-tight, yet ion-permeable, barrier that allows for proper maintaining proper serum salt balance and reabsorption. Once cleaved and released into urine, uromodulin inhibits crystal formation in the urine. Defects in this gene have been linked to kidney disease and stone formation. The immunogen used to raise the antibodies for this kit comprise the mature c-terminal portion of this protein. The N-terminal domains involved in polymerization were excluded. This protein does not share high sequence identity in mouse or human samples (87.4 and 74.5%, respectively) and, therefore, are not expected to cross-react.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.67" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "108 - 118 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "105 - 117 %", "average": "= 111" }, { "sample": "Urine", "range": "106 - 117 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "98 - 110 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "100 - 109 %", "average": "= 105" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab274405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB274405-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB274405-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB274405-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB274405-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB274405-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB274405-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB274405-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Uromodulin Lyophilized Recombinant Protein", "number":"AB274405-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Uromodulin Capture Antibody", "number":"AB274405-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB274405-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Uromodulin Detector Antibody", "number":"AB274405-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.
Biological function summary

Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.

Pathways

Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.

Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.
style
left-column

Product protocols

style
left-column

Target data

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.. Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Protects against urinary tract infections by binding to type 1 fimbriated E.coli. Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation. Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens.
See full target information Uromodulin
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com