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AB100787

Rat VEGF-A ELISA Kit

5

(1 Review)

|

(9 Publications)

Rat VEGF-A ELISA Kit is a Sandwich ELISA for the measurement of Rat VEGF-A in Rat in Cell/Tissue Extracts samples.

View Alternative Names

Vegf, Vegfa, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF

1 Images
Sandwich ELISA - Rat VEGF-A ELISA Kit (AB100787)
  • sELISA

Unknown

Sandwich ELISA - Rat VEGF-A ELISA Kit (AB100787)

Representative standard curve using ab100787

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

0.82 - 200 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat VEGF in cell lysates and tissue lysates.

This assay employs an antibody specific for Rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "81 - 102 %", "average": "= 92.67" }, { "sample": "Tissue Extracts", "range": "82 - 102 %", "average": "= 94.34" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100787-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100787-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100787-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VEGF Microplate (12 x 8 wells)", "number":"AB100787-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100787-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100787-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100787-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100787-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat VEGF Standard (lyophilized)", "number":"AB100787-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat VEGF", "number":"AB100787-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"120X HRP-Streptavidin Concentrate", "number":"AB100787-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).
See full target information Vegfa
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 24:88 PubMed38355510

2024

Apium extract alleviates indomethacin-induced gastric ulcers in rats via modulating the VEGF and IK-κB/NF-κB p65 signaling pathway: insights from in silico and in vivo investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Dalia H Abu-Baih,Alshymaa Abdel-Rahman Gomaa,Nada Mohamed Abdel-Wahab,Enas Reda Abdelaleem,Azza M Abdel Zaher,Noha F Hassan,Gerhard Bringmann,Usama Ramadan Abdelmohsen,Faisal H Altemani,Naseh A Algehainy,Fatma Alzahraa Mokhtar,Miada F Abdelwahab

Frontiers in cardiovascular medicine 9:981333 PubMed36818914

2023

Angiotensin receptor-neprilysin inhibitor improves coronary collateral perfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Kangbo Li,Victoria Kratzmann,Mengjun Dai,Nora Gatzke,Petra Rocic,Peter Bramlage,Olaf Grisk,Lubomir T Lubomirov,Meike Hoffmeister,Martin A Lauxmann,Oliver Ritter,Eva Buschmann,Michael Bader,Anja Bondke Persson,Ivo Buschmann,Philipp Hillmeister

Stem cell reports 17:276-288 PubMed35030322

2022

Physical exercise promotes integration of grafted cells and functional recovery in an acute stroke rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wu,Yiping Guo,Liying Zhang,Haiqing Zheng,Xi Chen,Mingyue Li,Qi Xing,Wenhao Huang,Zhenghui Su,Di Zhang,Xiaofen Zhong,Guangjin Pan,Xiquan Hu

Food & function 11:10413-10422 PubMed33237074

2020

Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Juhi Nema,Deepali Sundrani,Sadhana Joshi

Theranostics 10:1604-1618 PubMed32042325

2020

Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Dallan McMahon,Wendy Oakden,Kullervo Hynynen

Molecular and cellular biochemistry 461:159-170 PubMed31420792

2019

Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Vaishali Kasture,Deepali Sundrani,Surabhi Dalvi,Mayur Swamy,Anvita Kale,Sadhana Joshi

Oxidative medicine and cellular longevity 2017:5290161 PubMed28904737

2017

Anticataractogenesis and Antiretinopathy Effects of the Novel Protective Agent Containing the Combined Extract of Mango and Vietnamese Coriander in STZ-Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jintanaporn Wattanathorn,Paphaphat Thiraphatthanavong,Wipawee Thukham-Mee,Supaporn Muchimapura,Panakaporn Wannanond,Terdthai Tong-Un

Journal of neurochemistry 140:814-825 PubMed28002622

2017

Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen-glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Gina Hong,Kin Sing Stephen Lee,Bruce D Hammock,Debebe Gebremedhin,David R Harder,Raymond C Koehler,Adam Sapirstein

PloS one 9:e110109 PubMed25314292

2014

Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model.

Applications

EIA

Species

Rat

Eun Kyoung Lee,Young Joo Kim,Jin Young Kim,Hyun Beom Song,Hyeong Gon Yu
View all publications
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