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AB100786

Rat VEGF ELISA Kit

4

(1 Review)

|

(27 Publications)

Rat VEGF ELISA Kit is a Sandwich ELISA for the measurement of Rat VEGF in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Vegf, Vegfa, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF

4 Images
Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)
  • sELISA

Lab

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)

Sample results.

VEGF measured in A10 cell culture supernatant and biological fluids showing quantity (pg) per mL of tested sample. RBL-1, PC12 and SV40LT-SIV cell culture supernatants were also assayed for rat VEGF and measured > 200 pg/mL.

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)
  • sELISA

Lab

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)

VEGF measured in rat spleen and rat bone marrow showing quantity (pg) per mL of tested sample. Samples were diluted 9-27 fold.

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)
  • sELISA

Lab

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)
  • sELISA

Lab

Sandwich ELISA - Rat VEGF ELISA Kit (AB100786)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

0.82 - 200 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat VEGF in serum, plasma, cellculture supernatants.

This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 98.26" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 97.43" }, { "sample": "Plasma", "range": "85 - 104 %", "average": "= 96.42" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100786-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100786-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100786-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat VEGF Standard (lyophilized)", "number":"AB100786-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100786-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VEGF Microplate (12 x 8 wells)", "number":"AB100786-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"120X HRP-Streptavidin Concentrate", "number":"AB100786-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100786-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100786-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat VEGF", "number":"AB100786-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).
See full target information Vegfa
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:70 PubMed39940043

2025

Pericyte-derived extracellular vesicles improve vascular barrier function in sepsis via the Angpt1/PI3K/AKT pathway and pericyte recruitment: an in vivo and in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Sen Zhang,Ao Yang,Xi Luo,He-Nan Zhou,Yi-Yan Liu,Dai-Qin Bao,Jie Zhang,Jia-Tao Zang,Qing-Hui Li,Tao Li,Liang-Ming Liu

International journal of medical sciences 22:209-226 PubMed39744173

2025

Enhanced Bone Regeneration by Schwann Cells through Coupling of Osteogenesis and Angiogenesis via β-catenin signaling in a Preclinical Model of Distraction Osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wang,Yifan Yu,Rui Zhang,Jia Xu,Qinglin Kang

The Tohoku journal of experimental medicine 266:87-95 PubMed39443135

2024

Cornuside Ameliorates Diabetic Nephropathy Possibly by Regulating Angiogenesis and MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Xiang,Xin Li,Wei Hu

Cells 13: PubMed39056770

2024

Octadecaneuropeptide, ODN, Promotes Cell Survival against 6-OHDA-Induced Oxidative Stress and Apoptosis by Modulating the Expression of miR-34b, miR-29a, and miR-21in Cultured Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amine Bourzam,Yosra Hamdi,Seyma Bahdoudi,Karthi Duraisamy,Mouna El Mehdi,Magali Basille-Dugay,Omayma Dlimi,Maher Kharrat,Anne Vejux,Gérard Lizard,Taoufik Ghrairi,Benjamin Lefranc,David Vaudry,Jean A Boutin,Jérôme Leprince,Olfa Masmoudi-Kouki

Cells 12: PubMed38067139

2023

Repeated Intravenous Administration of Human Neural Stem Cells Producing Choline Acetyltransferase Exerts Anti-Aging Effects in Male F344 Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jangbeen Kyung,Dajeong Kim,Kyungha Shin,Dongsun Park,Soon-Cheol Hong,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

Frontiers in endocrinology 13:1013018 PubMed36714584

2023

Growth differentiation factor 10 induces angiogenesis to promote wound healing in rats with diabetic foot ulcers by activating TGF-β1/Smad3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Zhao,Jinmei Xu,Xu Han,Zheqi Zhang,Jiahui Qu,Zhifeng Cheng

Bone research 10:58 PubMed36031625

2022

Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuang Cui,Hangtian Wu,Ye Xiao,Ting Xu,Junjie Jia,Hancheng Lin,Rongmin Lin,Kun Chen,Yihuang Lin,Kaiqun Li,Xiaohu Wu,Changjun Li,Bin Yu

Stem cells international 2022:2976185 PubMed35571531

2022

Constructing Tissue-Engineered Dressing Membranes with Adipose-Derived Stem Cells and Acellular Dermal Matrix for Diabetic Wound Healing: A Comparative Study of Hypoxia- or Normoxia-Culture Modes.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Xin Zhao,Xin Shi,Can Chen,Yanpeng Cao,Jun Liu

Cells 11: PubMed35053347

2022

Combination Therapy of Placenta-Derived Mesenchymal Stem Cells with WKYMVm Promotes Hepatic Function in a Rat Model with Hepatic Disease via Vascular Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hye Jun,Sohae Park,Jae Yeon Kim,Ja-Yun Lim,Gyu Tae Park,Jae Ho Kim,Gi Jin Kim

Experimental and therapeutic medicine 22:1477 PubMed34765018

2021

Regulatory role of miRNA-23a in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Sun,Xuezheng Liu,Zhongfu Zuo
View all publications
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