Rat VEGFD ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat VEGFD with a sensitivity of 7.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 35.156 - 2250 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.35 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Alternative names
FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor
What's included?
Recommended products
Rat VEGFD ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat VEGFD with a sensitivity of 7.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 35.156 - 2250 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.35 pg/mL
- General recovery
- 89%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 6.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 89 | Range 84 - 92 % |
Sample type Cell culture media | Average % = 86 | Range 79 - 96 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat VEGFD SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGFD protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Rat VEGFD with 7.35 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.
- Biological function summary
Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.
- Pathways
The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.
- Associated diseases and disorders
Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.
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3 product images
Sandwich ELISA - Rat VEGFD ELISA Kit (ab309281)
50% mouse, human, and rat serum was measured in duplicate. Interpolation for all samples was performed using the rat standard curve. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat VEGFD ELISA Kit (ab309281)
Example of rat VEGFD standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat VEGFD ELISA Kit (ab309281)
Interpolated concentration of native VEGFD was measured in duplicate at different sample concentrations. Undiluted samples are 50% rat serum. The interpolated dilution factor corrected values to 100% sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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