Rat WISP1 ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat WISP1 - Intracellular in Cell Lysate samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
12.5 - 800 pg/mL
- Assay time
1h 30m
- Sensitivity
= 5.988 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity).
Alternative names
CCN family member 4, ELM-1, WNT1-inducible-signaling pathway protein 1, WISP-1, Wisp1, Elm1, Ccn4
What's included?
Recommended products
Rat WISP1 ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat WISP1 - Intracellular in Cell Lysate samples.
Alternative names
CCN family member 4, ELM-1, WNT1-inducible-signaling pathway protein 1, WISP-1, Wisp1, Elm1, Ccn4
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
12.5 - 800 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 5.988 pg/mL
- General recovery
105%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 3.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 4 | mean - | SD - | C.V. 5.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 105 | Range 101 - 109 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Rat WISP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of WISP1 protein in rat cell extract. Quantitate Rat WISP1 with 5.988 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.
- Biological function summary
WISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.
- Pathways
The network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.
- Associated diseases and disorders
WISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.
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3 product images
Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (ab290981)
Example of rat WISP1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (ab290981)
Interpolated concentration of native WISP1 was measured in duplicate at different sample concentrations. Undiluted samples are 75 μg/mL L6 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (ab290981)
Standard curve comparison between Rat WISP1 SimpleStep ELISA kit (ab290981) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.
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Product protocols
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