JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB290981

Rat WISP1 ELISA Kit - Intracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Rat WISP1 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Rat WISP1 with a sensitivity of 5.988 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Elm1, Wisp1, Ccn4, CCN family member 4, ELM-1, WNT1-inducible-signaling pathway protein 1, WISP-1

4 Images
Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)
  • sELISA

Supplier Data

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)

Standard curve comparison between Rat WISP1 SimpleStep ELISA kit (ab290981) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)
  • sELISA

Supplier Data

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)

Example of rat WISP1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)
  • sELISA

Supplier Data

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)

Interpolated concentration of native WISP1 was measured in duplicate at different sample concentrations. Undiluted samples are 75 μg/mL L6 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)
  • sELISA

Supplier Data

Sandwich ELISA - Rat WISP1 ELISA Kit - Intracellular (AB290981)

Representative image of Rat WISP1 ELISA Kit - Intracellular (ab290981)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Rat WISP1 - Intracellular Capture Antibody

- 10X Rat WISP1 - Intracellular Detector Antibody

- Rat WISP1 - Intracellular Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.988 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB285076

Anti-WISP1 antibody [EPR23996-174] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-WISP1 antibody [EPR23996-174] - BSA and Azide free (Detector) (AB285076)

  • 0
  • 0
Assay Kits

AB184857

Human GLP1 (7-36) ELISA Kit

Recombinant|SimpleStep

ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

  • 5
  • 3
Assay Kits

AB272101

Human Myeloperoxidase ELISA Kit

Recombinant|SimpleStep

ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

  • 0
  • 0
Assay Kits

AB204722

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Rat WISP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of WISP1 protein in rat cell extract. Quantitate Rat WISP1 with 5.988 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
style
left-column

Recovery

[ { "sample": "Cell Lysate", "range": "101 - 109 %", "average": "= 105" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab290981-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB290981-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB290981-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB290981-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB290981-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB290981-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB290981-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat WISP1 Capture Antibody", "number":"AB290981-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB290981-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB290981-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat WISP1 Lyophilized Recombinant Protein", "number":"AB290981-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB290981-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat WISP1 Detector Antibody", "number":"AB290981-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.
Biological function summary

WISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.

Pathways

The network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.

WISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.
style
left-column

Product protocols

style
left-column

Target data

Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity).
See full target information Ccn4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com