JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB178653

Anti-Rheumatoid Factor IgM ELISA Kit

5

(1 Review)

|

(4 Publications)

Anti-Rheumatoid Factor IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Rheumatoid Factor in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
1 Images
Functional Studies - Anti-Rheumatoid Factor IgM ELISA Kit (AB178653)
  • FuncS

Unknown

Functional Studies - Anti-Rheumatoid Factor IgM ELISA Kit (AB178653)

Representative Standard Curve using ab178653.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Assay type

Indirect

Sensitivity

< 0.5 U/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB256398

Rat CRP ELISA Kit (C-Reactive Protein)

Recombinant|SimpleStep

ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

  • 0
  • 0
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Assay Kits

AB212166

Human BDNF ELISA Kit

SimpleStep

ELISA - Human BDNF ELISA Kit (AB212166)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's anti-Rheumatoid Factor IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to Rheumatoid Factor IgM in Human serum or plasma.

The quantitative immunoenzymatic determination of IgM rheumatoid factors is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with Fc fragments of Human immunoglobulin G to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added. This conjugate binds to the captured rheumatoid factors. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of IgM rheumatoid factors in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178653-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Rheumatoid Factor Anti-IgM HRP Conjugate", "number":"AB178653-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB178653-CMP03", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Standard F - 300 U/mL", "number":"AB178653-CMP14", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM High Control", "number":"AB178653-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Standard B - 3 U/mL", "number":"AB178653-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Standard A - 0 U/mL", "number":"AB178653-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Standard C - 10 U/mL", "number":"AB178653-CMP11", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Standard D - 30 U/mL", "number":"AB178653-CMP12", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Standard E - 100 U/mL", "number":"AB178653-CMP13", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rheumatoid Factor IgM Low Control", "number":"AB178653-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB178653-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178653-CMP15", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178653-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IgG Fc Fragment Coated Microplate (12 x 8 wells)", "number":"AB178653-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178653-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178653-CMP16", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rheumatoid Factor IgM often referred to as IgM RF is an autoantibody that targets the Fc region of IgG immunoglobulins. It is primarily expressed in synovial fluid and blood plasma. The mass of the IgM RF antibody typically ranges between 970-1000 kDa. Its main role involves forming immune complexes by binding to IgG contributing to inflammation and tissue damage in autoimmune conditions.
Biological function summary

The role of Rheumatoid Factor IgM involves mediating immune responses by participating in the formation of antigen-antibody complexes. Although it is not a part of a complex by itself IgM RF can interact with other components of the immune system to exacerbate inflammatory reactions. A characteristic feature is its involvement in the activation of the complement system which plays a part in promoting inflammation and autoimmune processes.

Pathways

Rheumatoid Factor IgM influences processes in the complement cascade and the immune response pathway. It interacts with components such as C-reactive protein and immunoglobulin G both involved in the regulation of inflammatory responses. The activities of IgM RF within these pathways contribute to the enhancement and maintenance of chronic inflammation seen in certain autoimmune conditions.

Rheumatoid Factor IgM is most notably associated with rheumatoid arthritis and Sjögren's syndrome. It often correlates with disease severity and progression in rheumatoid arthritis by forming immune complexes that contribute to chronic joint inflammation and damage. In Sjögren's syndrome IgM RF along with anti-SS-A/Ro antibodies plays a role in the pathogenesis and facilitates glandular dysfunction and dryness. Its presence helps in diagnosis and prognosis of these diseases making it a significant biomarker in autoimmune disease management.
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2024:1995952 PubMed39669913

2024

Peptide BG From Bitter Gourd () Improves Adjuvant-Induced Arthritis by Modulating the Necroptosis/Neutrophil Extracellular Traps/Inflammation Axis and the Gut Microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Han,Yanan Xu,Suyila Qimuge,Changshan Wang,Xiulan Su

Biosensors 13: PubMed36979593

2023

Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Sánchez-Tirado,Lourdes Agüí,Marta Sánchez-Paniagua,Araceli González-Cortés,Beatriz López-Ruiz,Paloma Yáñez-Sedeño,José M Pingarrón

mAbs 11:1276-1288 PubMed31216930

2019

Antibody Fc engineering for enhanced neonatal Fc receptor binding and prolonged circulation half-life.

Applications

Unspecified application

Species

Unspecified reactive species

Brian C Mackness,Julie A Jaworski,Ekaterina Boudanova,Anna Park,Delphine Valente,Christine Mauriac,Olivier Pasquier,Thorsten Schmidt,Mostafa Kabiri,Abdullah Kandira,Katarina Radošević,Huawei Qiu

Molecular medicine reports 18:49-58 PubMed29749546

2018

Serum proteomic analysis of the anti‑arthritic effects of sinomenine on rats with collagen‑induced arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Qian,Zhiming Zhao,Wei Shang,Zhihan Xu,Beibei Zhang,Hui Cai
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com