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AB237654

anti-Rituximab ELISA Kit

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anti-Rituximab ELISA Kit is a Quantitative ELISA for the measurement of anti-Rituximab in Human in Biofluids samples.
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Sandwich ELISA - anti-Rituximab ELISA Kit (AB237654)
  • sELISA

Supplier Data

Sandwich ELISA - anti-Rituximab ELISA Kit (AB237654)

Typical Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Quantitative

Sensitivity

= 10 ng/mL

Range

75 - 500 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

anti-Rituximab ELISA Kit (ab237654) is designed to quantify/measure the antibody against Rituximab with high specificity and sensitivity in biological matrices.

This product is manufactured by BioVision, an Abcam company and was previously called E4385 BioSim™ anti-Rituximab (Mabthera®) (Human) ELISA Kit. E4385-100 is the same size as the 96 test size of ab237654.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 30" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 30" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 130 %", "average": null } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab237654-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Peroxidase Conjugate", "number":"AB237654-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB237654-CMP15", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rituximab Standard F", "number":"AB237654-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rituximab Standard E", "number":"AB237654-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rituximab Standard D", "number":"AB237654-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rituximab Standard C", "number":"AB237654-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rituximab Standard B", "number":"AB237654-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rituximab Standard A", "number":"AB237654-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Control - Low level", "number":"AB237654-CMP16", "productcode":"" }, { "size":"1 x 1 mL", "name":"Control - High level", "number":"AB237654-CMP17", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Buffer", "number":"AB237654-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB237654-CMP04", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plate sealers", "number":"AB237654-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB substrate", "number":"AB237654-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"Confirmation Reagent", "number":"AB237654-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash buffer (20X)", "number":"AB237654-CMP13", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rituximab also known as anti-CD20 is a monoclonal antibody that targets the CD20 protein expressed on the surface of B cells. The CD20 protein is a non-glycosylated phosphoprotein with a mass of approximately 33 to 37 kilodaltons. It is expressed in both normal and malignant B cells from the pre-B-cell stage through the mature B-cell stage. The protein is absent in pro-B cells and plasma cells which helps in the specific targeting of B cells without affecting the stem cells or other immune cells.
Biological function summary

Rituximab depletes B cells by inducing cell lysis through mechanisms like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Rituximab’s binding to CD20 leads to B cell apoptosis. As part of the immune system B cells produce antibodies; hence B cell depletion affects humoral immunity. However this depletion allows for the reduction of abnormal B cells in conditions like cancer and autoimmune diseases where aberrant B cell activity plays a harmful role.

Pathways

CD20 does not belong to a multi-component signaling complex but plays a role in calcium signaling in B cells. It modulates calcium influx which is critical for B cell activation and regulation. The pathways involving CD20 are associated with B-cell receptor signaling an essential mechanism regulating B cell survival and proliferation. Other proteins like SYK and BLNK are involved in the extended signaling cascade triggered by B-cell receptor activation.

Rituximab plays an important role in the treatment of B-cell non-Hodgkin lymphomas and rheumatoid arthritis. It targets and removes malignant B cells in lymphoma offering an effective therapy alongside traditional chemotherapies. In rheumatoid arthritis rituximab reduces B cell-mediated inflammation and tissue damage. Rituximab's specificity for CD20 allows it to reduce disease-associated B cells without destroying all B cells minimizing unintended consequences on the immune system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Anti-Rituximab
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
en
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB237640

Rituximab ELISA Kit

elisa-kits

rituximab-elisa-kit-ab237640

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