AB234573

S100B ELISA Kit

Recombinant
SimpleStep
What is this?

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(7 Publications)

S100B ELISA Kit is a single-wash 90-min Simplestep used to quantify S100B with a sensitivity of 139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

4 Images

ELISA

Supplier Data

ELISA - S100B ELISA Kit (AB234573)

sELISA

Unknown

Sandwich ELISA - S100B ELISA Kit (AB234573)

Interpolated concentrations of native S100B protein in human, mouse, and rat brain extracts based on 2 µg/mL, 60 µg/mL, and 1 µg/mL loads, respectively.

The concentrations of S100B were measured in duplicates, interpolated from the S100B standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - S100B ELISA Kit (AB234573)

Representative image of S100B ELISA Kit (ab234573)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X S100B ELISA Kit Capture Antibody

- 10X S100B Detector Antibody

- 10X S100B ELISA Kit Lyophilised Purified Protein (2 vials)

- S100B ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - S100B ELISA Kit (AB234573)

Example of S100B standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Mouse, Rat, Human

Assay type

Sandwich (quantitative)

Sensitivity

= 139 pg/mL

Range

0.31 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

S100B ELISA Kit ab234573 is a rapid single-wash 90-min Sandwich ELISA to measure S100B in tissue extracts. This SimpleStep sensitivity is 139 pg/mL.

How the assay works

S100B SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

S100B ELISA Kit ab234573 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

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Precision

[ { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "92 - 100 %", "average": "= 97" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234573-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234573-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234573-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234573-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234573-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234573-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234573-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human S100B Lyophilized Recombinant Protein", "number":"AB234573-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human S100B Capture Antibody", "number":"AB234573-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB234573-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human S100B Detector Antibody", "number":"AB234573-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234573-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234573-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.

Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.

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Product protocols

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Target data

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed : 20950652, PubMed : 6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed : 20950652, PubMed : 6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed : 20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed : 22399290).

See full target information S100B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell stress & chaperones 28:801-809 PubMed37608231

2023

Aucubin promotes activation of AMPK and alleviates cerebral ischemia/reperfusion injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Jing Zhao,Bo Zhao,Xiao Bai,Shuang Zhang,Rui Xu

Neurobiology of disease 176:105964 PubMed36526090

2022

TNF and IL6/Jak2 signaling pathways are the main contributors of the glia-derived neuroinflammation present in Lafora disease, a fatal form of progressive myoclonus epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Rubio,Rosa Viana,Mireia Moreno-Estellés,Ángela Campos-Rodríguez,Pascual Sanz

BMC neurology 22:334 PubMed36068493

2022

Predictive nomogram for soft robotic hand rehabilitation of patients with intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Jin,Wei Jiang,Qing Bao,Wenfeng Wei,Wenqing Jiang

Biosensors 12: PubMed35323442

2022

Towards a Point-of-Care (POC) Diagnostic Platform for the Multiplex Electrochemiluminescent (ECL) Sensing of Mild Traumatic Brain Injury (mTBI) Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Milica Jović,Denis Prim,Edis Saini,Marc Emil Pfeifer

International journal of critical illness and injury science 11:246-252 PubMed35070915

2021

Statin therapy associated with decreased neuronal injury measured by serum S100β levels in patients with acute ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hayder M Al-Kuraishy,Ali I Al-Gareeb,Marwa Thaier Naji

Experimental and therapeutic medicine 20:275 PubMed33200000

2020

Effect of dexmedetomidine combined with etomidate on IL-17A and S-100β expression levels in rats with postoperative cognitive dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Yu,Yunfang Xie

Experimental and therapeutic medicine 20:112 PubMed32989390

2020

Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Pan,Xu Zheng,Liang Wang,Qian Chen,Qi Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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