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AB285278

Salmonella Typhi IgG ELISA Kit

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Salmonella Typhi IgG ELISA Kit is a ELISA for the measurement of Salmonella Typhi IgG in in Biofluids samples.

Key facts

Sample types

Plasma, Serum

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This ELISA assay (ab285278, E4678) could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.

This product is manufactured by BioVision, an Abcam company and was previously called E4678 Salmonella Typhi IgG ELISA Kit. E4678-100 is the same size as the 96 test size of ab285278.

Salmonella typhi is the causative agent of typhoid fever a contagious infection of the intestines that affects the whole body. In developing countries, typhoid often occurs in epidemics. Most people in the United States get typhoid as a result of visiting another country where the food or water supply has been contaminated. Symptoms usually start 1 to 3 weeks after exposure to the bacteria. Symptoms include: high fever, headache, sore throat, vomiting, diarrhea, skin rash and weakness. The symptoms may take 2 weeks or more to go away. Typhoid is spread when a person drinks or eats food and water contaminated by human waste (stool or urine) containing Salmonella typhi bacteria. A person who no longer has symptoms may still transmit the bacteria as a carrier. Testing for immunoglobulin G (IgG), IgA, and IgM antilipopolysaccharide (LPS) of Salmonella typhi antibodies by enzyme-linked immunosorbent assay (ELISA) showed that the levels of all three classes of immunoglobulin anti-LPS of S. typhi were higher in typhoid patients than in healthy or febrile nontyphoidal groups. The ELISA assay was much more sensitive and specific than any combination of the Widal test, and hence it could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285278-96Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Positive Control", "number":"AB285278-CMP04", "productcode":"" }, { "size":"2 x 25 mL", "name":"Sample Diluent", "number":"AB285278-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Calibrator", "number":"AB285278-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Negative Control", "number":"AB285278-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash buffer", "number":"AB285278-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Enzyme conjugate", "number":"AB285278-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB285278-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate", "number":"AB285278-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB285278-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Salmonella Typhi IgG antibody plays an essential role in immune response against Salmonella Typhi infection. Salmonella Typhi sometimes known as Salmonella enterica serovar Typhi causes typhoid fever in humans. This IgG antibody usually produced by the immune system following infection or vaccination briefly circulates in the bloodstream to recognize and neutralize the specific antigens presented by the pathogen. Salmonella Typhi IgG’s mass varies due to its immunoglobulin nature and can differ slightly among individuals. Expression occurs in response to infection prominently present in serum after initial immune response.
Biological function summary

The Salmonella Typhi IgG antibody functions as a critical player in identifying and neutralizing the pathogenic actions of Salmonella Typhi. It does not form a complex but it specifically binds to the antigens of the pathogen facilitating opsonization and macrophage engulfment. This antibody-mediated response is vital for long-term immune protection often evaluated through laboratory methods such as ELISA for Salmonella detection. ELISA for Salmonella remains a popular method to quantify Salmonella Typhi IgG levels helping in clinical diagnosis and monitoring of immune status post-vaccination or infection.

Pathways

The production and action of Salmonella Typhi IgG occur within the adaptive immune system. IgG interacts notably with pathways involving antigen processing and presentation. This interaction involves other proteins such as major histocompatibility complex (MHC) molecules and T-cell receptors critical for driving a robust immune response. During antigen presentation antigen fragments bind to MHC molecules presented to helper T cells which in turn stimulate B cells to produce Salmonella-specific IgG antibodies.

Salmonella Typhi IgG is directly associated with typhoid fever a life-threatening illness. Presence and quantification of Salmonella IgG in serum can help diagnose and evaluate previous exposure or vaccination effectiveness against Salmonella Typhi. Another disease associated is paratyphoid fever caused by related strains like Salmonella Paratyphi where cross-reaction of antibodies might occur. Proteins like MHC and T-cell receptors again play roles in shaping the immune strategy against these diseases navigating the complexities of antigen recognition and immune activation.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

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