Serotonin ELISA Kit is a Competitive ELISA kit for the measurement of Serotonin in Urine, Plasma, Serum, Platelets samples.
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Publications
- Nature communications 14:74062023Mesenchymal stromal cells alleviate depressive and anxiety-like behaviors via a lung vagal-to-brain axis in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Huang,Weijun Huang,Junzhe Yi,Yiwen Deng,Ruijie Li,Jieying Chen,Jiahao Shi,Yuan Qiu,Tao Wang,Xiaoyong Chen,Xiaoran Zhang,Andy Peng XiangPubMed 37973914
- Frontiers in microbiology 14:11749682023Multi-strain probiotics alleviate loperamide-induced constipation by adjusting the microbiome, serotonin, and short-chain fatty acids in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Ju Jeong,Raja Ganesan,Yoo-Jeong Jin,Hee Jin Park,Byeong Hyun Min,Min Kyo Jeong,Sang Jun Yoon,Mi Ran Choi,Jieun Choi,Ji Hyun Moon,Uigi Min,Jong-Hyun Lim,Do Yup Lee,Sang Hak Han,Young Lim Ham,Byung-Yong Kim,Ki Tae SukPubMed 37333632
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Serum, Platelets
- Assay type
Competitive
- Range
0.49 - 500 ng/mL
- Assay time
3h
- Sensitivity
= 0.293 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
5 hydroxytryptamine, 5HT
What's included?
Recommended products
Serotonin ELISA Kit is a Competitive ELISA kit for the measurement of Serotonin in Urine, Plasma, Serum, Platelets samples.
Alternative names
5 hydroxytryptamine, 5HT
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Serum, Platelets
- Assay type
Competitive
- Range
0.49 - 500 ng/mL
- Assay time
3h
- Assay Platform
Microplate
- Sensitivity
= 0.293 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample 13.5 ng/mL Spike | n 20 | mean - | SD - | C.V. 11 |
Sample 346.1 ng/mL Spike | n 20 | mean - | SD - | C.V. 4.2 |
Sample 53.8 ng/mL Spike | n 20 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample 13.2 ng/mL Spike | n 0 | mean - | SD - | C.V. 12.7 |
Sample 358.0 ng/mL Spike | n 0 | mean - | SD - | C.V. 16.2 |
Sample 53.9 ng/mL Spike | n 0 | mean - | SD - | C.V. 18.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Platelets | Average % = 107 | Range 106 - 109 % |
Sample type Serum | Average % = 91 | Range 83 - 95 % |
Sample type Citrate plasma | Average % = 95 | Range 83 - 102 % |
Sample type Urine | Average % = 101 | Range 91 - 107 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Serotonin ELISA kit is a competitive ELISA kit designed for the accurate quantitative measurement of Serotonin in Human Serum, Platelets, Plasma and Urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Cross reactivities:
| Compound | Cross Reactivity |
| N-Acetyl Serotonin | 17% |
| 5-Hydroxy-L-tryptophan | 0.4% |
| Tryptamine | 0.1% |
| 5-Hydroxyindoleacetic acid | 0.03% |
| Melatonin | 0.01% |
| Tyramine | <0.004% |
| Tryptophan | <0.004% |
Supplementary info
- Activity summary
Serotonin also known as 5-hydroxytryptamine (5-HT) is a neurotransmitter mainly involved in modulating mood emotion and behavior. It weighs approximately 176.2 Da and appears in several body tissues including the central nervous system gastrointestinal tract and blood platelets. Neurons within the raphe nuclei of the brainstem predominantly synthesize serotonin influencing various physiological processes. Its mechanical role involves binding to and activating specific serotonin receptors classified into seven different types each mediating distinct signaling pathways.
- Biological function summary
Serotonin regulates multiple bodily functions beyond mood such as appetite sleep and circadian rhythm. It forms part of a complex system interacting with various receptor subtypes which include the anti-serotonergic pathways inhibiting its action. Each receptor subtype leads to different downstream effects in cells contributing to the diversity of serotonin's biological roles. For research purposes scientists often use an ELISA kit to measure serotonin levels and this can influence the ELISA test cost based on the specificity and sensitivity required.
- Pathways
Serotonin participates in both the serotonergic and dopaminergic pathways illustrating its central role in neurotransmission. The serotonergic pathway is essential for transmitting nerve impulses while its interaction with the dopaminergic system significantly affects mood and reward-related behaviors. Key proteins such as tryptophan hydroxylase for serotonin synthesis and the vesicular monoamine transporter are involved within these pathways and may alter serotonin's functional availability.
- Associated diseases and disorders
Serotonin is closely linked to depression and anxiety disorders where its imbalance can lead to mood disturbances. Antidepressant treatments often target serotonin receptors or its transporters restoring its levels within synapses. Serotonin's connection to these conditions extends through its relationship with proteins like the serotonin transporter (SERT) which recycles serotonin in the synaptic cleft. Understanding these dynamics presents potential targets for interventions and anti-serotonergic agents may offer therapeutic options for managing such disorders.
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3 product images
Competitive ELISA - Serotonin ELISA Kit (ab133053)
Serotonin measured (averages of undil., 1:3, 1:9 & 1:27) in biological fluids (Neuro2a an Growth med. averaged from 1:2 and 1:4) showing quantity (ng) per mL of tested sample.
Competitive ELISA - Serotonin ELISA Kit (ab133053)
Representative Standard Curve using ab133053.
Competitive ELISA - Serotonin ELISA Kit (ab133053)
Typical Parrallelism Graph.
Dose-response curves from Human platelets and Human serum diluted into assay buffer were compared to the Serotonin standard curve. The parallel response indicates the standard effectively mimics the native protein.
Downloads
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