The Serotonin (5-HT) ELISA kit (colorimetric) is a competitive ELISA designed to quantify serotonin (5-hydroxytryptamine, 5-HT) in serum, plasma, urine, and platelets with a sensitivity of 0.293 ng/mL.
- Wide dynamic range – quantifies 0.49–500 ng/mL
- No acylation required for standards and sample
- Assay platform – microplate for easy setup and reliable results
- Cited in over 30 publications
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Publications
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- Nature communications 14:74062023Mesenchymal stromal cells alleviate depressive and anxiety-like behaviors via a lung vagal-to-brain axis in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Huang,Weijun Huang,Junzhe Yi,Yiwen Deng,Ruijie Li,Jieying Chen,Jiahao Shi,Yuan Qiu,Tao Wang,Xiaoyong Chen,Xiaoran Zhang,Andy Peng XiangPubMed 37973914
- Frontiers in microbiology 14:11749682023Multi-strain probiotics alleviate loperamide-induced constipation by adjusting the microbiome, serotonin, and short-chain fatty acids in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Ju Jeong,Raja Ganesan,Yoo-Jeong Jin,Hee Jin Park,Byeong Hyun Min,Min Kyo Jeong,Sang Jun Yoon,Mi Ran Choi,Jieun Choi,Ji Hyun Moon,Uigi Min,Jong-Hyun Lim,Do Yup Lee,Sang Hak Han,Young Lim Ham,Byung-Yong Kim,Ki Tae SukPubMed 37333632
- BMC cancer 23:2672023Exposure to nonanoic acid alters small intestinal neuroendocrine tumor phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBilal Almobarak,Vishal Amlani,Linda Inge,Tobias Hofving,Andreas Muth,Ola Nilsson,Martin Johansson,Yvonne Arvidsson,Erik EliasPubMed 36959559
- Frontiers in pharmacology 13:10626502023Differentiated HT22 cells as a novel model for screening of serotonin reuptake inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuhee Lim,Yeojin Bang,Kyeong-Man Kim,Hyun Jin ChoiPubMed 36703746
- Cells 11:2022Modulation of Neuroendocrine and Immunological Biomarkers Following Rehabilitation in Sarcopenic Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederica Piancone,Francesca La Rosa,Ivana Marventano,Ambra Hernis,Rossella Miglioli,Fabio Trecate,Marina Saresella,Mario ClericiPubMed 36010554
- Journal of ginseng research 46:819-8292022Anxiolytic effect of Korean Red Ginseng through upregulation of serotonin and GABA transmission and BDNF expression in immobilized mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBich Phuong Bui,Phuong Linh Nguyen,Ha Thi Thu Do,Jungsook ChoPubMed 36312738
- Frontiers in immunology 13:9113812022Resveratrol Alleviating the Ovarian Function Under Oxidative Stress by Alternating Microbiota Related Tryptophan-Kynurenine Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianping Wang,Ru Jia,Pietro Celi,Yong Zhuo,Xuemei Ding,Qiufeng Zeng,Shiping Bai,Shengyu Xu,Huadong Yin,Li Lv,Keying ZhangPubMed 35911670
- Nutrients 14:2022Circulating Human Serum Metabolites Derived from the Intake of a Saffron Extract (Safr'Inside) Protect Neurons from Oxidative Stress: Consideration for Depressive Disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabien Wauquier,Line Boutin-Wittrant,Line Pourtau,David Gaudout,Benjamin Moras,Adeline Vignault,Camille Monchaux De Oliveira,Julien Gabaston,Carole Vaysse,Karène Bertrand,Hélène Abrous,Lucile Capuron,Nathalie Castanon,David Vauzour,Véronique Roux,Nicolas Macian,Gisèle Pickering,Yohann WittrantPubMed 35406124
- International journal of molecular sciences 23:2022Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyeoung-Hwa Kim,Kyung-Ah LeePubMed 35008991
- International journal of molecular sciences 22:2021Alterations in Metabolome and Microbiome Associated with an Early Stress Stage in Male Wistar Rats: A Multi-Omics Approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Hernandez-Baixauli,Pere Puigbò,Nerea Abasolo,Hector Palacios-Jordan,Elisabet Foguet-Romero,David Suñol,Mar Galofré,Antoni Caimari,Laura Baselga-Escudero,Josep M Del Bas,Miquel MuleroPubMed 34884735
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum, Platelets
- Assay type
- Competitive
- Range
- 0.49 - 500 ng/mL
- Assay time
- 3h
- Sensitivity
- = 0.293 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
5 hydroxytryptamine, 5HT
What's included?
Recommended products
The Serotonin (5-HT) ELISA kit (colorimetric) is a competitive ELISA designed to quantify serotonin (5-hydroxytryptamine, 5-HT) in serum, plasma, urine, and platelets with a sensitivity of 0.293 ng/mL.
- Wide dynamic range – quantifies 0.49–500 ng/mL
- No acylation required for standards and sample
- Assay platform – microplate for easy setup and reliable results
- Cited in over 30 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum, Platelets
- Assay type
- Competitive
- Range
- 0.49 - 500 ng/mL
- Assay time
- 3h
- Assay Platform
- Microplate
- Sensitivity
- = 0.293 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample 13.5 ng/mL Spike | n 20 | C.V. 11 |
Sample 346.1 ng/mL Spike | n 20 | C.V. 4.2 |
Sample 53.8 ng/mL Spike | n 20 | C.V. 5.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample 13.2 ng/mL Spike | n 0 | C.V. 12.7 |
Sample 358.0 ng/mL Spike | n 0 | C.V. 16.2 |
Sample 53.9 ng/mL Spike | n 0 | C.V. 18.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Platelets | Average % = 107 | Range 106 - 109 % |
Sample type Serum | Average % = 91 | Range 83 - 95 % |
Sample type Citrate plasma | Average % = 95 | Range 83 - 102 % |
Sample type Urine | Average % = 101 | Range 91 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Serotonin ELISA Kit ab133053 is a competitive ELISA to measure Serotonin (5 hydroxytryptamine) in Urine, Plasma, Serum, Platelets with a sensitivity of 0.293 ng/mL.
How the assay works
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.
Assay specificity
Cross reactivity:
- N-Acetyl Serotonin : 17%
- 5-Hydroxy-L-tryptophan: 0.4%
- Tryptamine: 0.1%
- 5-Hydroxyindoleacetic acid: 0.03%
- Melatonin: 0.01%
- Tyramine: <0.004%
- Tryptophan: <0.004%
Serotonin ELISA Kit ab133053 protocol summary
1. Add samples and standards to the wells
2. Add prepared labeled AP-conjugate to appropriate wells
3. Add Serotonin antibody to appropriate wells. Incubate at room temperature
4. Add pNpp substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Serotonin ELISA kit ab133053
Serotonin ELISA kit ab133053 has been used in publications to study the role of peripheral MSCs in major depressive disorder (1), rehabilitation effects on inflammation and sarcopenia (2), and the correlation between seratonin levels and heart failure (3).
References:
(1)Andy Peng Xiang et al. 2023 PMID: 37973914,
(2)Mario Clerici et al. 2022 PMID: 36010554,
(3)Roselyn Barbara Rose'Meyer et al. 2020 PMID: 34645867
Related and recommended products
Serotonin ELISA kit ab133053 is commonly used to study behavioral and neurodegenerative disoders. Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Other kits for measuring neurotransmitters are:
- Dopamine ELISA Kit Dopamine ELISA Kit ab285238
- Glutamate Assay Kit Glutamate Assay Kit ab83389
- Norepinephrine ELISA Kit Norepinephrine ELISA Kit ab287789
- Adrenaline ELISA Kit Adrenaline ELISA Kit ab287788
- Human Acetylcholine ELISA Kit Human Acetylcholine ELISA Kit ab287811
- Human GABA ELISA Kit Human GABA ELISA Kit ab287792
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Cross reactivities:
| Compound | Cross Reactivity |
| N-Acetyl Serotonin | 17% |
| 5-Hydroxy-L-tryptophan | 0.4% |
| Tryptamine | 0.1% |
| 5-Hydroxyindoleacetic acid | 0.03% |
| Melatonin | 0.01% |
| Tyramine | <0.004% |
| Tryptophan | <0.004% |
Serotonin ELISA Kit ab133053 is a competitive ELISA to measure Serotonin (5 hydroxytryptamine) in Urine, Plasma, Serum, Platelets with a sensitivity of 0.293 ng/mL.
How the assay works
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.
Assay specificity
Cross reactivity:
- N-Acetyl Serotonin : 17%
- 5-Hydroxy-L-tryptophan: 0.4%
- Tryptamine: 0.1%
- 5-Hydroxyindoleacetic acid: 0.03%
- Melatonin: 0.01%
- Tyramine: <0.004%
- Tryptophan: <0.004%
Serotonin ELISA Kit ab133053 protocol summary
1. Add samples and standards to the wells
2. Add prepared labeled AP-conjugate to appropriate wells
3. Add Serotonin antibody to appropriate wells. Incubate at room temperature
4. Add pNpp substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Serotonin ELISA kit ab133053
Serotonin ELISA kit ab133053 has been used in publications to study the role of peripheral MSCs in major depressive disorder (1), rehabilitation effects on inflammation and sarcopenia (2), and the correlation between serotonin levels and heart failure (3).
References:
(1)Andy Peng Xiang et al. 2023 PMID: 37973914,
(2)Mario Clerici et al. 2022 PMID: 36010554, (3)Roselyn Barbara Rose'Meyer et al. 2020 PMID: 34645867
Related and recommended products
Serotonin ELISA kit ab133053 is commonly used to study behavioral and neurodegenerative disorders. Serotonin is a neurotransmitter.
Other kits for measuring neurotransmitters are:
- Dopamine ELISA Kit Dopamine ELISA Kit ab285238
- Glutamate Assay Kit Glutamate Assay Kit ab83389
- Norepinephrine ELISA Kit Norepinephrine ELISA Kit ab287789
- Adrenaline ELISA Kit Adrenaline ELISA Kit ab287788
- Human Acetylcholine ELISA Kit Human Acetylcholine ELISA Kit ab287811
- Human GABA ELISA Kit Human GABA ELISA Kit ab287792
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serotonin also known as 5-hydroxytryptamine (5-HT) is a neurotransmitter mainly involved in modulating mood emotion and behavior. It weighs approximately 176.2 Da and appears in several body tissues including the central nervous system gastrointestinal tract and blood platelets. Neurons within the raphe nuclei of the brainstem predominantly synthesize serotonin influencing various physiological processes. Its mechanical role involves binding to and activating specific serotonin receptors classified into seven different types each mediating distinct signaling pathways.
- Biological function summary
Serotonin regulates multiple bodily functions beyond mood such as appetite sleep and circadian rhythm. It forms part of a complex system interacting with various receptor subtypes which include the anti-serotonergic pathways inhibiting its action. Each receptor subtype leads to different downstream effects in cells contributing to the diversity of serotonin's biological roles. For research purposes scientists often use an ELISA kit to measure serotonin levels and this can influence the ELISA test cost based on the specificity and sensitivity required.
- Pathways
Serotonin participates in both the serotonergic and dopaminergic pathways illustrating its central role in neurotransmission. The serotonergic pathway is essential for transmitting nerve impulses while its interaction with the dopaminergic system significantly affects mood and reward-related behaviors. Key proteins such as tryptophan hydroxylase for serotonin synthesis and the vesicular monoamine transporter are involved within these pathways and may alter serotonin's functional availability.
- Associated diseases and disorders
Serotonin is closely linked to depression and anxiety disorders where its imbalance can lead to mood disturbances. Antidepressant treatments often target serotonin receptors or its transporters restoring its levels within synapses. Serotonin's connection to these conditions extends through its relationship with proteins like the serotonin transporter (SERT) which recycles serotonin in the synaptic cleft. Understanding these dynamics presents potential targets for interventions and anti-serotonergic agents may offer therapeutic options for managing such disorders.
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3 product images
Competitive ELISA - Serotonin ELISA Kit (ab133053)
Serotonin measured (averages of undil., 1:3, 1:9 & 1:27) in biological fluids (Neuro2a an Growth med. averaged from 1:2 and 1:4) showing quantity (ng) per mL of tested sample.
Competitive ELISA - Serotonin ELISA Kit (ab133053)
Representative Standard Curve using ab133053.
Competitive ELISA - Serotonin ELISA Kit (ab133053)
Typical Parrallelism Graph.
Dose-response curves from Human platelets and Human serum diluted into assay buffer were compared to the Serotonin standard curve. The parallel response indicates the standard effectively mimics the native protein.
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