Serotonin ELISA Kit

Product details

Serotonin ELISA Kit ab133053 is a competitive ELISA to measure Serotonin (5 hydroxytryptamine) in Urine, Plasma, Serum, Platelets with a sensitivity of 0.293 ng/mL.

How the assay works
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.

Assay specificity
Cross reactivity:
- N-Acetyl Serotonin : 17%
- 5-Hydroxy-L-tryptophan: 0.4%
- Tryptamine: 0.1%
- 5-Hydroxyindoleacetic acid: 0.03%
- Melatonin: 0.01%
- Tyramine: <0.004%
- Tryptophan: <0.004%

Serotonin ELISA Kit ab133053 protocol summary
1. Add samples and standards to the wells
2. Add prepared labeled AP-conjugate to appropriate wells
3. Add Serotonin antibody to appropriate wells. Incubate at room temperature
4. Add pNpp substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately

How other researchers are using Serotonin ELISA kit ab133053
Serotonin ELISA kit ab133053 has been used in publications to study the role of peripheral MSCs in major depressive disorder ⁽¹⁾, rehabilitation effects on inflammation and sarcopenia ⁽²⁾, and the correlation between seratonin levels and heart failure ⁽³⁾.

References:
⁽¹⁾Andy Peng Xiang et al. 2023 PMID: 37973914,
⁽²⁾Mario Clerici et al. 2022 PMID: 36010554,
⁽³⁾Roselyn Barbara Rose'Meyer et al. 2020 PMID: 34645867

Related and recommended products
Serotonin ELISA kit ab133053 is commonly used to study behavioral and neurodegenerative disoders. Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.

Other kits for measuring neurotransmitters are:
- Dopamine ELISA Kit ab285238
- Glutamate Assay Kit ab83389
- Norepinephrine ELISA Kit ab287789
- Adrenaline ELISA Kit ab287788
- Human Acetylcholine ELISA Kit ab287811
- Human GABA ELISA Kit ab287792

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.

Cross reactivities: